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首页> 外文期刊>BMC Genomics >Transcriptome profiling provides insights into dormancy release during cold storage of Lilium pumilum
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Transcriptome profiling provides insights into dormancy release during cold storage of Lilium pumilum

机译：转录组谱分析提供了对Lilum pumilum冷藏期间休眠释放的见解

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Bulbs of the ornamental flower Lilium pumilum enter a period of dormancy after flowering in spring, and require exposure to cold for a period of time in order to release dormancy. Previous studies focused mainly on anatomical, physiological and biochemical changes during dormancy release. There are no dormancy studies of the northern cold-hardy wild species of Lilium at the molecular level. This study observed bulb cell and starch granule ultrastructures during cold storage; and analysed the transcriptome using sequencing. The combination of morphological and transcriptomic methods provides valuable insights into dormancy release during cold storage of Lilium pumilum. Ultrastructural changes reflected dormancy release during cold storage of the bulbs. We compared gene expression levels among samples at 0 (S1 stage), 30 (S2 stage), 60 (S3 stage) and 90 (S4 stage) d of cold storage, with 0 d as the control. The data showed that some regulatory pathways such as carbohydrate metabolism and plant hormone signal transduction were activated to break dormancy. Some differentially expressed genes (DEGs) related to antioxidant activity, epigenetic modification and transcription factors were induced to respond to low temperature conditions. These genes constituted a complex regulatory mechanism of dormancy release. Cytological data related to dormancy regulation was obtained through histomorphological observation; transcriptome sequencing provided comprehensive sequences and digital gene expression tag profiling (DGE) data, and bulb cell ultrastructural changes were closely related to DEGs. The novel Lilium pumilum genetic information from this study provides a reference for the regulation of dormancy by genetic engineering using molecular biology tools.
机译：观赏花百合鳞茎的鳞茎在春季开花后进入休眠期，并且需要暴露于寒冷中一段时间​​才能释放休眠。先前的研究主要集中在休眠释放过程中的解剖，生理和生化变化。没有在分子水平上对北方百合的野生耐寒物种进行休眠研究。本研究观察了冷藏过程中鳞茎细胞和淀粉颗粒的超微结构。并使用测序分析了转录组。形态学和转录组学方法的结合提供了对百合冷藏期间休眠释放的有价值的见解。超微结构的变化反映了灯泡冷藏期间休眠的释放。我们比较了0 d（S1阶段），30（S2阶段），60（S3阶段）和90（S4阶段）冷藏样品在0 d作为对照的基因表达水平。数据表明，一些调节途径如糖代谢和植物激素信号转导被激活以打破休眠。诱导了一些与抗氧化活性，表观遗传修饰和转录因子相关的差异表达基因（DEG），以响应低温条件。这些基因构成了休眠释放的复杂调控机制。通过组织形态学观察获得与休眠调节有关的细胞学数据。转录组测序提供了全面的序列和数字基因表达标签图谱（DGE）数据，并且鳞茎细胞的超微结构变化与DEGs密切相关。来自这项研究的新颖的百合铝遗传信息为使用分子生物学工具进行基因工程的休眠调控提供了参考。

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