...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Genomics >Large-scale identification of wheat genes resistant to cereal cyst nematode Heterodera avenae using comparative transcriptomic analysis
【24h】

Large-scale identification of wheat genes resistant to cereal cyst nematode Heterodera avenae using comparative transcriptomic analysis

机译：使用比较转录组分析技术大规模鉴定抗谷类囊肿线虫异型小麦的小麦基因

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cereal cyst nematode Heterodera avenae, an important soil-borne pathogen in wheat, causes numerous annual yield losses worldwide, and use of resistant cultivars is the best strategy for control. However, target genes are not readily available for breeding resistant cultivars. Therefore, comparative transcriptomic analyses were performed to identify more applicable resistance genes for cultivar breeding. The developing nematodes within roots were stained with acid fuchsin solution. Transcriptome assemblies and redundancy filteration were obtained by Trinity, TGI Clustering Tool and BLASTN, respectively. Gene Ontology annotation was yielded by Blast2GO program, and metabolic pathways of transcripts were analyzed by Path_finder. The ROS levels were determined by luminol-chemiluminescence assay. The transcriptional gene expression profiles were obtained by quantitative RT-PCR. The RNA-sequencing was performed using an incompatible wheat cultivar VP1620 and a compatible control cultivar WEN19 infected with H. avenae at 24?h, 3 d and 8 d. Infection assays showed that VP1620 failed to block penetration of H. avenae but disturbed the transition of developmental stages, leading to a significant reduction in cyst formation. Two types of expression profiles were established to predict candidate resistance genes after developing a novel strategy to generate clean RNA-seq data by removing the transcripts of H. avenae within the raw data before assembly. Using the uncoordinated expression profiles with transcript abundance as a standard, 424 candidate resistance genes were identified, including 302 overlapping genes and 122 VP1620-specific genes. Genes with similar expression patterns were further classified according to the scales of changed transcript abundances, and 182 genes were rescued as supplementary candidate resistance genes. Functional characterizations revealed that diverse defense-related pathways were responsible for wheat resistance against H. avenae. Moreover, phospholipase was involved in many defense-related pathways and localized in the connection position. Furthermore, strong bursts of reactive oxygen species (ROS) within VP1620 roots infected with H. avenae were induced at 24?h and 3 d, and eight ROS-producing genes were significantly upregulated, including three class III peroxidase and five lipoxygenase genes. Large-scale identification of wheat resistance genes were processed by comparative transcriptomic analysis. Functional characterization showed that phospholipases associated with ROS production played vital roles in early defense responses to H. avenae via involvement in diverse defense-related pathways as a hub switch. This study is the first to investigate the early defense responses of wheat against H. avenae, not only provides applicable candidate resistance genes for breeding novel wheat cultivars, but also enables a better understanding of the defense mechanisms of wheat against H. avenae.
机译：谷物囊肿线虫Heterodera avenae是小麦中一种重要的土壤传播病原体，在世界范围内每年造成大量产量损失，而使用抗性品种是最佳的控制策略。但是，靶基因不易用于抗性育种。因此，进行了比较转录组学分析，以鉴定更多适用于品种育种的抗性基因。用酸性品红溶液将根内发育中的线虫染色。转录组组装和冗余过滤分别通过Trinity，TGI Clustering Tool和BLASTN获得。通过Blast2GO程序产生基因本体注释，并通过Path_finder分析转录物的代谢途径。 ROS水平通过鲁米诺化学发光测定法测定。通过定量RT-PCR获得转录基因表达谱。使用不相容的小麦品种VP1620和相容的对照品种WEN19（在24？h，3 d和8 d时感染了燕麦）的RNA测序。感染检测表明，VP1620未能阻止青霉菌的穿透，但干扰了发育阶段的过渡，从而导致囊肿形成的显着减少。建立了两种表达谱以预测候选抗性基因，这是在开发通过在组装前去除原始数据中的avenus H. avenae转录本的新颖策略来生成干净的RNA-seq数据后预测的候选抗性基因。使用转录本丰度作为标准的不协调表达谱，鉴定出424个候选抗性基因，包括302个重叠基因和122个VP1620特异性基因。根据转录物丰度变化的程度进一步分类表达模式相似的基因，并拯救了182个基因作为补充候选抗性基因。功能特征表明，与防御相关的途径多种多样，是小麦对H. avenae的抗性的原因。此外，磷脂酶参与许多与防御相关的途径，并位于连接位置。此外，在24？h和3 d诱导了被avenae感染的VP1620根中强烈的活性氧（ROS）爆发，并且显着上调了8个ROS产生基因，包括3个III类过氧化物酶和5个脂氧合酶基因。通过比较转录组分析处理了小麦抗性基因的大规模鉴定。功能表征表明，与ROS产生相关的磷脂酶通过参与多种与防御相关的途径作为枢纽开关，在对燕麦的早期防御反应中起着至关重要的作用。这项研究是第一个研究小麦对H. avenae的早期防御反应的方法，不仅为育种新小麦品种提供了适用的候选抗性基因，而且使人们对小麦对H. avenae的防御机制有了更好的了解。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号