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首页> 外文期刊>British Journal of Cancer >Lymphocyte subsets in tumour of patients with undifferentiated nasopharyngeal carcinoma: presence of lymphocytes with the phenotype of activated T cells
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Lymphocyte subsets in tumour of patients with undifferentiated nasopharyngeal carcinoma: presence of lymphocytes with the phenotype of activated T cells

机译：未分化鼻咽癌患者肿瘤中的淋巴细胞亚群：存在具有活化T细胞表型的淋巴细胞

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We have analyzed lymphocytes infiltrating nasopharyngeal carcinomas, using a combination of immunoperoxidase staining of frozen and paraffin-embedded sections, and immunofluorescence on lymphocyte suspensions recovered from teased tumours. A panel of monoclonal antibodies was used to define lymphocytic subsets on frozen sections of 14 different tumours. The vast majority of peri- and intra-tumoral lymphocytes were stained by OKT3 antibody. In 8 sections, T4 positive cells were largely predominant, while T8 positive cells were the majority in three sections. Twenty-nine paraffin-embedded sections from other NPC patients stained with HNK-1 antibody showed a variable percentage of positive cells reaching 6 to 15% in nine patients. Most HNK-1 positive cells had the morphology of large granular lymphocytes typical of natural killer cells. Double staining experiments on lymphocytes isolated from 7 tumours revealed a constant presence of T3 positive, HLA-DR positive lymphocytes (from 6 to 29% of mononuclear cells), and of lymphocytes coexpressing the T3 and the Tac (IL-2 receptor) antigens (from 5 to 12% of mononuclear cells). Lymphocytes with a phenotype of activated T-cells are thus constantly found in NPC tumours.
机译：我们使用冷冻和石蜡包埋切片的免疫过氧化物酶染色，以及对取自肿瘤的淋巴细胞悬液进行免疫荧光分析，分析了浸润鼻咽癌的淋巴细胞。使用一组单克隆抗体来定义14种不同肿瘤的冷冻切片上的淋巴细胞亚群。 OKT3抗体对肿瘤周围和肿瘤内的绝大多数淋巴细胞染色。在8个切片中，T4阳性细胞主要占优势，而在三个切片中T8阳性细胞占多数。来自其他NPC患者的29块石蜡包埋的切片用HNK-1抗体染色，显示9例患者中阳性细胞的可变百分比达到6％至15％。大多数HNK-1阳性细胞具有自然杀伤细胞典型的大颗粒淋巴细胞的形态。对从7种肿瘤中分离出的淋巴细胞进行的双重染色实验表明，恒定存在T3阳性，HLA-DR阳性淋巴细胞（占单核细胞的6％到29％）以及共表达T3和Tac（IL-2受体）抗原的淋巴细胞（ 5至12％的单核细胞）。因此在NPC肿瘤中不断发现具有活化T细胞表型的淋巴细胞。

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