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首页> 外文期刊>Clinical Chemistry: Journal of the American Association for Clinical Chemists >Carbohydrate-deficient transferrin and false-positive results for alcohol abuse in primary biliary cirrhosis: differential diagnosis by detection of mitochondrial autoantibodies.
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Carbohydrate-deficient transferrin and false-positive results for alcohol abuse in primary biliary cirrhosis: differential diagnosis by detection of mitochondrial autoantibodies.

机译：缺乏碳水化合物的转铁蛋白和原发性胆汁性肝硬化中酒精滥用的假阳性结果：通过检测线粒体自身抗体进行鉴别诊断。

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摘要

Primary biliary cirrhosis (PBC) is one of the few nonalcohol-induced liver pathologies that causes false positives in assays of carbohydrate-deficient transferrin (CDT) for diagnosing alcohol abuse. CDT was quantified by isoelectric focusing-immunoblotting-laser densitometry (IEF-IB-LD) analysis of serum from 117 women: 57 PBC patients, 20 alcohol abusers, and 40 healthy donors. Only 5% (3 of 57) of PBC patients were positive at the densitometric cutoff value chosen ( 90% specificity). Serum samples from 15 PBC patients were further evaluated by IEF-IB-LD and CDTect chromatography-RIA. Receiver-operating characteristic (ROC) analysis showed that IEF-IB-LD better discriminated between PBC and alcohol abuse than CDTect did. By ROC analysis, mitochondrial autoantibodies to pyruvate dehydrogenase antigen M2 detected by enzyme immunoassay yielded optimal test performance for diagnosing PBC. Of six patients falsely positive for CDT by CDTect, five (83%) tested M2-positive. Thus, abnormal CDT results should be further evaluated by mitochondrial antibody testing in patients with findings compatible with PBC.
机译：原发性胆汁性肝硬化（PBC）是少数非酒精性肝病之一，可在诊断碳水化合物滥用的碳水化合物缺乏型转铁蛋​​白（CDT）分析中引起假阳性。 CDT通过等电聚焦-免疫印迹-激光密度法（IEF-IB-LD）对117名妇女的血清进行定量分析：57名PBC患者，20名酗酒者和40名健康捐献者。在选择的光密度测定临界值（> 90％特异性）时，只有5％（57个中的3个）的PBC患者为阳性。通过IEF-IB-LD和CDTect色谱-RIA进一步评估了15名PBC患者的血清样本。接受者操作特征（ROC）分析显示，与CDTect相比，IEF-IB-LD更好地区分了PBC和酒精滥用。通过ROC分析，通过酶联免疫法检测到的针对丙酮酸脱氢酶抗原M2的线粒体自身抗体产生了诊断PBC的最佳测试性能。通过CDTect对CDT假阳性的6名患者中，有5名（83％）检测出M2阳性。因此，对于发现与PBC相容的患者，应通过线粒体抗体检测进一步评估CDT异常结果。

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