Concentration and purification of enteroviruses by membrane chromatography.

M Henderson; C Wallis; J L Melnick

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Concentration and purification of enteroviruses by membrane chromatography.

机译：通过膜色谱法浓缩和纯化肠病毒。

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A simple procedure for the concentration and partial purification of enteroviruses from tissue culture harvests is described. After removal of acid-precipitating components with a cationic detergent, the detergent and most membrane-coating components were removed by treatment with a cationic-exchange resin. The resin effluent was then acidified, and the virus was adsorbed to epoxy-fiberglass membranes. Virus was then eluted with pH 11.5 glycine-NaOH buffer. Since this eluate contains no orgcentrated simply by acidifying the eluate and passing it through a smaller membrane than that used for the first concentration. As high as 500-fold concentrations can be achieved, with a high efficiency of recovery.

机译：描述了从组织培养物收获物中浓缩和部分纯化肠病毒的简单程序。用阳离子去污剂除去酸沉淀组分后，用阳离子交换树脂处理除去去污剂和大多数膜包衣组分。然后酸化树脂流出物，并将病毒吸附到环氧玻璃纤维膜上。然后用pH 11.5甘氨酸-NaOH缓冲液洗脱病毒。由于该洗脱液不包含任何酸化成分，只需通过酸化该洗脱液并使之穿过比用于第一浓缩液的膜更小的膜即可。可以实现高达500倍的浓度，而且回收效率很高。

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《Applied and Environmental Microbiology》 |1976年第5期|共5页
作者
M Henderson; C Wallis; J L Melnick;
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1. Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR. [J] . K J Schwab, R De Leon, M D Sobsey Applied and Environmental Microbiology . 1995,第2期

机译：浓缩和纯化水样中的牛肉提取物模拟洗脱液，用于通过逆转录PCR检测肠病毒，甲型肝炎病毒和诺沃克病毒。
2. Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR. [J] . K J Schwab, R De Leon, M D Sobsey Applied and Environmental Microbiology . 1995,第2期

机译：浓缩和纯化水样中的牛肉提取物模拟洗脱液，用于通过逆转录PCR检测肠病毒，甲型肝炎病毒和诺沃克病毒。
3. Purification of biomolecules combining ATPS and membrane chromatography. (Special Issue: Advances in bioseparations for food and bioprocessing.) [J] . Puthirasigamany M., Hamm I., Winssen F. A. van, Food and Bioproducts Processing. Transactions of the Institution of Chemical Engineers, Part C . 2014,第2期

机译：结合ATPS和膜色谱法纯化生物分子。（特刊：食品和生物加工的生物分离研究进展。）
4. Concentration and Purification of Densonucleosis Virus by Tangential Membrane Filtration and by Ion Exchange Membranes [C] . Peter Czermak, David L. Grzenia, Anne Wolf, European Society of Animal Cell Technology . 2010

机译：通过切向膜过滤和离子交换膜浓缩和纯化抗体杂物杂散病毒
5. Purification of Inducible Oxide Synthase and Calmodulin Complex Using Nickel Ion Affinity, 2' -5' -ADP- Sepharose and Gel Filtration Column Chromatography. [D] . Kongchan, Natee. 2009

机译：使用镍离子亲和力，2'-5'-ADP-琼脂糖凝胶电泳和凝胶过滤柱色谱法纯化可诱导的氧化物合酶和钙调蛋白复合物。
6. Concentration and purification of enteroviruses by membrane chromatography. [O] . M Henderson, C Wallis, J L Melnick 1976

机译：通过膜色谱法浓缩和纯化肠病毒。
7. Purification of the chloroplast-membrane dicyclohexylcarbodi-imide-binding proteolipid by ion-exchange chromatography. [O] . Sigrist-Nelson, K, Azzi, A 1979

机译：通过离子交换色谱法纯化叶绿体膜二环己基碳二亚胺结合的蛋白脂质。
8. Purification of Mouse Monoclonal Immunoglobulin M by Ion-Exchange Liquid Chromatography. (Reannouncement with New Availability Information). [R] . Rothman, S. W., Gentry, M. K., Gawne, R. D., 1989

机译：离子交换液相色谱法纯化小鼠单克隆免疫球蛋白m. （重新公布新的可用性信息）。

Concentration and purification of enteroviruses by membrane chromatography.

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