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首页> 外文会议>European Society of Animal Cell Technology >Concentration and Purification of Densonucleosis Virus by Tangential Membrane Filtration and by Ion Exchange Membranes
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Concentration and Purification of Densonucleosis Virus by Tangential Membrane Filtration and by Ion Exchange Membranes

机译：通过切向膜过滤和离子交换膜浓缩和纯化抗体杂物杂散病毒

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In gene therapy and vaccine production, large-scale purification of virus vectors is often essential. In the manufacture of biopharmaceuticals, validation of virus clearance is critical. Tangential flow filtration is well established in the biotechnology industry for the purification and concentration of proteins. Also chromatography is widely used in downstream processing and the replacement of resins by membranes as chromatographic supports is of interest to overcome limitations associated with resin-based chromatography. Purification of the Aedes aegyptl densonucleosis virus (AeDNV) using tangential flow filtration and also using anion and cation exchange membranes was investigated. The results showed that 30, 50, and 100 kDa membranes reject the virus particles in contrast to 300 kDa membranes. The permeate flux is strongly affected by the used medium. AeDNV particles may be adsorbed most effective by strongly basic anion exchange membranes.
机译：在基因治疗和疫苗生产中，病毒载体的大规模纯化通常是必不可少的。在制造生物制药中，病毒间隙的验证至关重要。在生物技术行业中，切向流过滤纯化和浓缩蛋白质。还广泛用于下游加工的色谱法，并通过膜更换树脂作为色谱载体，克服与基于树脂的色谱相关的限制。研究了使用切向流过滤和使用阴离子和阳离子交换膜的AEDES AEGYPT1杂膦病毒（AEDNV）的纯化。结果表明，30,50和100kDa膜与300kDa膜相比，抑制病毒颗粒。渗透助焊剂受到二手介质的强烈影响。通过强碱的阴离子交换膜吸附AEDNV颗粒最有效。

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