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首页> 外文期刊>Applied and Environmental Microbiology >Genetic Diversity in Bradyrhizobium japonicum Serogroup 123 and Its Relation to Genotype-Specific Nodulation of Soybean
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Genetic Diversity in Bradyrhizobium japonicum Serogroup 123 and Its Relation to Genotype-Specific Nodulation of Soybean

机译：大豆根瘤菌血清群123的遗传多样性及其与大豆基因型特异结瘤的关系

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摘要

The genetic diversity among 20 field isolates of Bradyrhizobium japonicum serogroup 123 was examined by using restriction endonuclease digestions, one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total cell proteins, Southern hybridization analysis of nif and nod genes, and intrinsic antibiotic resistance profiles. All of the isolates were previously separated into three broad nodulation classes (low, medium, and high) based on their ability to form symbioses with specific soybean genotypes. Results of our studies indicate that there is a relationship between these three genotype-specific nodulation classes and groupings that have been made based on genomic DNA digestion patterns, sodium dodecyl sulfate-protein profiles, and Southern hybridizations to a nifHD gene probe. Intrinsic antibiotic resistance profiles and nodAB gene hybridizations were not useful in determining interrelationships between isolates and nodulation classes. Southern hybridizations revealed that two of the isolates had reiterated nod genes; however, there was no correlation between the presence of extra nodAB genes and the nodulation classes or symbiotic performance on permissive soybean genotypes. Hybridizations with the nif gene probe indicated that there is a relationship among serogroup, nodulation class, and the physical organization of the genome.
机译：通过限制性内切酶消化，总细胞蛋白的一维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，nif和nod基因的Southern杂交分析以及内在的抗生素抗性图谱，研究了日本短臂螺旋体血清群123的20个野外分离株的遗传多样性。根据所有分离物形成具有特定大豆基因型共生酶的能力，先前已将其分为三大类瘤（低，中和高）。我们的研究结果表明，这三种基因型特异性结瘤类别与基于基因组DNA消化模式，十二烷基硫酸钠-蛋白质谱以及与nifHD基因探针的Southern杂交所建立的分组之间存在关联。固有的抗生素抗性概况和nodAB基因杂交在确定分离株和结瘤类别之间的相互关系方面无用。 Southern杂交显示，其中两个分离株重申了nod基因。然而，多余的nodAB基因的存在与根瘤分类或共生大豆基因型表现之间没有相关性。与nif基因探针的杂交表明，血清群，结节类别和基因组的物理组织之间存在关联。

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