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首页> 外文期刊>Applied and Environmental Microbiology >Lectin-Like Constituents of Foods Which React with Components of Serum, Saliva, and Streptococcus mutans
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Lectin-Like Constituents of Foods Which React with Components of Serum, Saliva, and Streptococcus mutans

机译：与血清，唾液和变形链球菌成分发生反应的食物中类似于凝集素的成分

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Hot and cold aqueous extracts were prepared from 22 commonly ingested fruits, vegetables, and seeds. When tested by agar diffusion, extracts from 13 and 10 of the foods formed precipitin bands with samples of normal rabbit serum and human saliva, respectively; extracts from four of the foods also reacted with antigen extracts of strains of Streptococcus mutans. When added to rabbit antiserum, extracts from 18 of 21 foods tested inhibited reactivity with antigen extracts derived from S. mutans MT3. Extracts from 16 foods agglutinated whole S. mutans cells, whereas those from 10 foods agglutinated human erythrocytes of blood types A and B. The lectin-like activities of extracts which reacted with human saliva were studied further. Pretreatment of saliva-coated hydroxyapatite (S-HA) beads with extracts of bananas, coconuts, carrots, alfalfa, and sunflower seeds markedly reduced the subsequent adsorption of S. mutans MT3. Pretreatment of S-HA with banana extract also strongly inhibited adsorption of S. mutans H12 and S. sanguis C1, but it had little effect on attachment of Actinomyces naeslundii L13 or A. viscosus LY7. Absorption experiments indicated that the component(s) in banana extract responsible for inhibiting streptococcal adsorption to S-HA was identical to that which bound to human erythrocytes. The banana hemagglutinin exhibited highest activity between pH 7 and 8, and it was inhibited by high concentrations of glucosamine, galactosamine, and, to a lesser extent, mannosamine. Other sugars tested had no effect. The selective bacterial adsorption-inhibiting effect noted for banana extract was also observed in studies with purified lectins. Thus, pretreating S-HA with wheat germ agglutinin and concanavalin A inhibited adsorption of S. mutans MT3 cells, whereas peanut agglutinin, Ulex agglutinin, Dolichos agglutinin, and soybean agglutinin had little effect; none of these lectins affected attachment of A. viscosus LY7. Collectively, the observations suggest that many foods contain lectins which can interact with components of human saliva and S. mutans cells. Because of their potential to influence host-parasite interactions in the mouth and elsewhere in the gastrointestinal canal, these reactions warrant further study.
机译：从22种通常摄入的水果，蔬菜和种子中提取冷热水提取物。用琼脂扩散法检测时，从13种和10种食物中提取的食物分别与正常兔血清和人唾液样品形成沉淀蛋白条带。四种食物中的提取物也与变形链球菌菌株的抗原提取物反应。当添加到兔抗血清中时，测试的21种食物中有18种的提取物会抑制与变形链球菌MT3的抗原提取物的反应性。 16种食物的提取物凝集了整个变形链球菌，而10种食物的提取物凝集了A型和B型血人红细胞。进一步研究了与人唾液反应的提取物的凝集素样活性。用香蕉，椰子，胡萝卜，苜蓿和向日葵种子的提取物对唾液包覆的羟基磷灰石（S-HA）珠进行预处理，可显着减少变形链球菌MT3的后续吸附。用香蕉提取物预处理S-HA还可强烈抑制变形链球菌H12和血红链球菌C1的吸附，但对内生放线菌L13或黏稠曲霉LY7的附着几乎没有影响。吸收实验表明，香蕉提取物中抑制链球菌对S-HA吸附的成分与与人红细胞结合的成分相同。香蕉血凝素在pH 7和8之间表现出最高的活性，并被高浓度的葡萄糖胺，半乳糖胺和少量的甘露糖胺抑制。测试的其他糖没有作用。在纯化的凝集素的研究中也观察到了香蕉提取物的选择性细菌吸附抑制作用。因此，用小麦胚芽凝集素和伴刀豆球蛋白A预处理S-HA会抑制变形链球菌MT3细胞的吸附，而花生凝集素，Ulex凝集素，Dolichos凝集素和大豆凝集素的作用很小。这些凝集素均不影响粘滞曲霉LY7的附着。总的来说，这些观察结果表明，许多食物都含有凝集素，这些凝集素可以与人唾液和变形链球菌细胞的成分相互作用。由于它们可能影响口腔和胃肠道其他部位的宿主-寄生虫相互作用，因此这些反应值得进一步研究。

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