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首页> 外文期刊>Applied and Environmental Microbiology >Automation of the Limulus amoebocyte lysate test by using the Abbott MS-2 microbiology system.
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Automation of the Limulus amoebocyte lysate test by using the Abbott MS-2 microbiology system.

机译：通过使用Abbott MS-2微生物系统自动进行Li变形细胞溶解物测试。

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A rapid, automated method for the performance of the Limulus amoebocyte lysate endotoxin assay has been developed by using the Abbott MS-2 Microbiology System. This instrument automatically determines sequential changes in the optical density of up to 176 samples at 1- or 5-min increments during a 1-h assay period. Graphic representation of optical density changes can be viewed on a cathode-ray tube or reproduced by using a hard-copy printer. Limulus amoebocyte lysate preparations that were obtained from different commercial producers and that had similar endotoxin sensitivities by the conventional gelation method varied somewhat in reactivity when determinations were based upon rate changes in optical density. Lysates from Associates of Cape Cod, Difco Laboratories, and M. A. Bioproducts were the most readily adaptable to the MS-2 System. Use of the MS-2 system increased the sensitivity of these preparations from 60- to 250-fold, and as little as 1 pg/ml was detected. Adaptation of the MS-2 instrument for this purpose provides an objective, reproducible, automated method for the performance of Limulus amoebocyte lysate tests on a variety of fluids.
机译：通过使用Abbott MS-2微生物系统，已经开发了一种快速，自动化的方法来进行Li变形细胞溶胞产物内毒素测定。该仪器可在1小时的测试期间以1或5分钟的增量自动确定多达176个样品的光密度的顺序变化。可以在阴极射线管上查看光密度变化的图形表示，也可以使用硬拷贝打印机进行复制。当基于光密度的速率变化进行测定时，从不同的商业生产商处获得的and血变形细胞裂解物制剂通过常规胶凝方法具有相似的内毒素敏感性，其反应性有所不同。来自科德角，Difco实验室和M. A.生物制品公司的裂解物最容易适应MS-2系统。 MS-2系统的使用将这些制剂的灵敏度从60倍提高到250倍，并且检测到低至1 pg / ml。为此，对MS-2仪器进行调整可提供一种客观，可重现的自动化方法，可对各种液体进行Li变形细胞溶解物测试。

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