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首页> 外文期刊>Applied and Environmental Microbiology >Detection of Plasmid Transfer from Pseudomonas fluorescens to Indigenous Bacteria in Soil by Using Bacteriophage φR2f for Donor Counterselection
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Detection of Plasmid Transfer from Pseudomonas fluorescens to Indigenous Bacteria in Soil by Using Bacteriophage φR2f for Donor Counterselection

机译：利用噬菌体φR2f进行供体反选择检测荧光假单胞菌向土壤中的原生细菌转移质粒

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摘要

The transfer of a genetically marked derivative of plasmid RP4, RP4p, from Pseudomonas fluorescens to members of the indigenous microflora of the wheat rhizosphere was studied by using a bacteriophage that specifically lyses the donor strain and a specific eukaryotic marker on the plasmid. Transfer of RP4p to the wheat rhizosphere microflora was observed, and the number of transconjugants detected was approximately 103 transconjugants per g of soil when 107 donor cells per g of soil were added; transfer in the corresponding bulk soil was slightly above the limit of detection. All of the indigenous transconjugants which we analyzed contained a 60-kb plasmid and were able to transfer this plasmid to a Nxr RprP. fluorescens recipient strain. The indigenous transconjugants were identified as belonging to Pseudomonas spp., Enterobacter spp., Comamonas spp., and Alcaligenes spp.
机译：通过使用特异性裂解供体菌株的噬菌体和质粒上的特定真核生物标记物，研究了从荧光假单胞菌中获得遗传标记的质粒RP4，RP4p衍生物从小麦假根菌的原生菌群转移的过程。观察到RP4p向小麦根际微生物区系的转移，当每克土壤添加107个供体细胞时，每克土壤中检测到的转结合剂数量约为103个。在相应的块状土壤中的迁移略高于检测极限。我们分析的所有本地转导结合体均含有60kb质粒，并且能够将该质粒转移至Nxr RprP。荧光受体菌株。鉴定出的本地转导结合体属于假单胞菌属，肠杆菌属，Comamonas属和产碱菌。

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