• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied and Environmental Microbiology >Reinvestigation of Brevibacterium sp. Strain KY-4313 as a Source of Canthaxanthin
【24h】

Reinvestigation of Brevibacterium sp. Strain KY-4313 as a Source of Canthaxanthin

机译：短杆菌属的再研究。菌株KY-4313作为角黄素的来源

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The hydrocarbon-utilizing Brevibacterium sp. strain KY-4313 was reevaluated for its potential to produce canthaxanthin, a carotenoid pigment of strong commercial interest. Three approaches were used to optimize the canthaxanthin yield from this organism, i.e., the preparation of mutants, the addition of supposedly carotenogenic chemicals to the growth medium, and growth promotion. Following treatment of the parent strain with N-nitrosomethylurea, a presumed mutant was isolated which showed a 32% increase in cellular canthaxanthin content. No effective carotenogenic chemicals were found in connection with hydrocarbon fermentations, in which mainly growth promotion through periodic medium renewal proved conducive to enhanced pigment production. Carotenogenesis could be stimulated in brain heart infusion broth by adding alcohols or retinol. Improved growth in this medium was generally not associated with higher canthaxanthin yields. Both superior growth and pigment levels were obtained in a newly designed medium based on fumaric acid-molasses. The maximum yields of canthaxanthin in shake flasks were (in milligrams per liter) 4.2 (brain heart infusion broth plus propanol-zinc sulfate), 3.6 (hydrocarbon medium), and 9.3 (fumaric acid-molasses), which represent a significant improvement over the originally reported optimal result (1 mg/liter). The corresponding yields of echinenone, the direct precursor of canthaxanthin, were 1.2, 1.6, and 2.3 mg/liter, respectively. Two-liter hydrocarbon batch fermentations involving medium renewal maximally produced 7.2 mg of canthaxanthin and 3.7 mg of echinenone per liter.
机译：利用烃的短杆菌属。重新评估了菌株KY-4313的潜力，以生产角黄素（一种具有很强商业价值的类胡萝卜素颜料）。三种方法被用来优化从该生物体得到的角黄素产量，即，制备突变体，向生长培养基中添加所谓的类胡萝卜素化学物质，以及促进生长。用N-亚硝基甲基脲处理亲本菌株后，分离出一个推测的突变体，该突变体显示出细胞角黄素含量增加了32％。没有发现与烃发酵有关的有效的致龋化学物质，其中主要通过定期培养基更新促进生长被证明有助于增加色素的产生。通过添加酒精或视黄醇可以刺激大脑心脏输液肉汤中的类胡萝卜素生成。在这种培养基中生长的改善通常与更高的角黄素产量无关。在基于富马酸糖蜜的新设计培养基中，均获得了优异的生长和色素含量。摇瓶中角黄素的最大产量为（毫克/升）4.2（脑心浸液肉汤加丙醇-硫酸锌），3.6（烃培养基）和9.3（富马酸-糖蜜），与最初报告的最佳结果（1毫克/升）。角黄素的直接前体海胆烯酮的相应产量分别为1.2、1.6和2.3 mg / L。每升涉及介质更新的两升烃分批发酵最多可产生7.2 mg角黄素和3.7 mg棘烯酮。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号