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Patterns of Reactivity between a Panel of Monoclonal Antibodies and Forage Rhizobium Strains

机译：一组单克隆抗体和饲草根瘤菌菌株之间的反应性模式

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摘要

A panel of 11 monoclonal antibodies raised against vegetative cells of Rhizobium leguminosarum biovar trifolii or Rhizobium meliloti was tested by enzyme-linked immunosorbent assay for reactivity with 47 strains of R. leguminosarum biovar trifolii and 60 strains of R. meliloti. The goal of the study was to define the degree of specificity associated with each antibody and to gain an understanding of the amount of antigenic diversity found among the strains and between the species. Each antibody was tested against each Rhizobium strain in four forms: washed steamed cells, washed unsteamed cells, cell-free culture broth, and nodule squash material. Each antibody showed a different pattern of reactivity among the 107 strains. One of each of the antibodies developed against R. meliloti and R. leguminosarum biovar trifolii reacted in a highly specific manner with cells or antigen from the immunogenic strain only. Nine of the antibodies recognized secreted as well as cellular antigen from many of the strains. Analysis of patterns of reactivity between the 107 strains and the 11 antibodies separated the strains into 28 groups of which 12 were represented by one strain only.
机译：通过酶联免疫吸附试验测试了一组针对豆科根瘤菌生物变种或苜蓿根瘤菌营养细胞产生的11种单克隆抗体与47株豆科根瘤菌和60株苜蓿根瘤菌的反应性。该研究的目的是确定与每种抗体相关的特异性程度，并了解在菌株之间以及物种之间发现的抗原多样性的数量。针对每种根瘤菌菌株以四种形式测试每种抗体：洗涤过的蒸干细胞，洗涤过的未蒸干细胞，无细胞培养液和根瘤南瓜材料。每种抗体在107个菌株中显示出不同的反应模式。针对meliloti和R. leguminosarum biovar trifolii开发的每种抗体中的一种以高度特异性的方式与仅来自免疫原性菌株的细胞或抗原反应。从许多菌株中识别出九种抗体以及分泌的抗原和细胞抗原。通过分析107株和11种抗体之间的反应性模式，可将菌株分为28组，其中12个仅由一个菌株代表。

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