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首页> 外文期刊>Scandinavian journal of immunology. >The frequent expansion of a subpopulation of B cells that express RF-associated cross-reactive idiotypes: evidence from analysis of a panel autoreactive monoclonal antibodies.
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The frequent expansion of a subpopulation of B cells that express RF-associated cross-reactive idiotypes: evidence from analysis of a panel autoreactive monoclonal antibodies.

机译：表达RF相关交叉反应独特型的B细胞亚群的频繁扩增：来自一组自身反应性单克隆抗体分析的证据。

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摘要

Preferential expression of VH gene segments is evident within the adult human primary B-cell repertoire. The repertoire may be influenced by genetic factors, e.g. VH gene segment polymorphisms, or in a temporal manner due to the exposure to environmental antigens. The molecular characteristics of 15 autoreactive human monoclonal antibodies (MoAbs) are reported. All antibodies were of the IgM isotype, and 12 of the 15 were polyreactive and included rheumatoid factor type specificity, i.e. reactivity with IgG. Nine of the 15 MoAbs are products of VH3 gene segments, as evidenced by staphylococcal protein A binding; four of these express the cross-reactive idiotype recognized by the mouse MoAb 3H7 and are thus products of the VH26 gene segment. One of the five remaining VH3 gene products expresses the cross-reactive idiotypes recognized by the mouse MoAbs B6 and D12. V-gene family usage, determined by polymerase chain reaction (PCR) amplification of cDNA and further hybridization with family-specific oligonucleotide probes, confirmed the cross-reactive idiotype studies and showed that only VH3-gene-encoded proteins bound staphylococcal protein A. Five of the six non-VH3 gene segment products express the cross-reactive idiotype recognized by the mouse MoAb LC1 and could be assumed to be products of the VH4.21 gene segment; however, one human MoAb is shown to be the product of a VH2 gene segment. This is interesting because it turns LC1 from being an anti-cross-reactive idiotype antibody into an anticlan reagent.
机译：VH基因片段的优先表达在成人原代B细胞库中很明显。曲目可能会受到遗传因素的影响，例如VH基因片段多态性，或由于暴露于环境抗原而在时间上的方式。报告了15种自身反应性人类单克隆抗体（MoAb）的分子特征。所有抗体均为IgM同种型，在15种抗体中有12种具有多反应性，并包括类风湿因子类型的特异性，即与IgG的反应性。 15种MoAb中有9种是VH3基因片段的产物，如葡萄球菌A蛋白结合所证明的。其中四个表达小鼠MoAb 3H7识别的交叉反应独特型，因此是VH26基因区段的产物。其余五个VH3基因产物之一表达了小鼠MoAbs B6和D12识别的交叉反应独特型。 V基因家族的使用，是通过cDNA的聚合酶链反应（PCR）扩增以及与家族特异性寡核苷酸探针的进一步杂交确定的，证实了交叉反应的独特型研究，并表明只有VH3基因编码的蛋白结合葡萄球菌蛋白A。5六个非VH3基因片段产物中有六个表达了小鼠MoAb LC1识别的交叉反应独特型，可以被认为是VH4.21基因片段的产物。然而，已显示一种人MoAb是VH2基因区段的产物。这很有趣，因为它将LC1从抗交叉反应的独特型抗体变成了anticlan试剂。

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