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首页> 外文期刊>Applied and Environmental Microbiology >Nitrogen Cycling and Community Structure of Proteobacterial β-Subgroup Ammonia-Oxidizing Bacteria within Polluted Marine Fish Farm Sediments
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Nitrogen Cycling and Community Structure of Proteobacterial β-Subgroup Ammonia-Oxidizing Bacteria within Polluted Marine Fish Farm Sediments

机译：污染的海水养鱼场沉积物中细菌细菌的β-亚族氨氧化细菌的氮循环和群落结构

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A multidisciplinary approach was used to study the effects of pollution from a marine fish farm on nitrification rates and on the community structure of ammonia-oxidizing bacteria in the underlying sediment. Organic content, ammonium concentrations, nitrification rates, and ammonia oxidizer most-probable-number counts were determined in samples of sediment collected from beneath a fish cage and on a transect at 20 and 40 m from the cage. The data suggest that nitrogen cycling was significantly disrupted directly beneath the fish cage, with inhibition of nitrification and denitrification. Although visual examination indicated some slight changes in sediment appearance at 20 m, all other measurements were similar to those obtained at 40 m, where the sediment was considered pristine. The community structures of proteobacterial β-subgroup ammonia-oxidizing bacteria at the sampling sites were compared by PCR amplification of 16S ribosomal DNA (rDNA), using primers which target this group. PCR products were analyzed by denaturing gradient gel electrophoresis (DGGE) and with oligonucleotide hybridization probes specific for different ammonia oxidizers. A DGGE doublet observed in PCR products from the highly polluted fish cage sediment sample was present at a lower intensity in the 20-m sample but was absent from the pristine 40-m sample station. Band migration, hybridization, and sequencing demonstrated that the doublet corresponded to a marineNitrosomonas group which was originally observed in 16S rDNA clone libraries prepared from the same sediment samples but with different PCR primers. Our data suggest that this novelNitrosomonas subgroup was selected for within polluted fish farm sediments and that the relative abundance of this group was influenced by the extent of pollution.
机译：一种多学科的方法被用来研究海洋鱼类养殖场的污染对硝化速率和底层沉积物中氨氧化细菌群落结构的影响。确定了从鱼笼下面以及距笼子20和40 m处的样线收集的沉积物样品中的有机物含量，铵浓度，硝化速率和氨氧化剂的最可能数量计数。数据表明，氮循环在鱼笼正下方被严重破坏，从而抑制了硝化作用和反硝化作用。尽管目视检查显示在20 m处沉积物外观有些细微变化，但所有其他测量值都与在40 m处获得的测量结果相似，在40 m处沉积物被认为是原始的。通过PCR扩增16S核糖体DNA（rDNA），并使用靶向该组的引物，比较了采样位点的细菌β-亚组氨氧化细菌的群落结构。通过变性梯度凝胶电泳（DGGE）并使用对不同氨氧化剂特异的寡核苷酸杂交探针分析PCR产物。在高度污染的鱼笼沉积物样品的PCR产品中观察到的DGGE双峰在20 m样品中的强度较低，但在40 m原始样品台中却没有。谱带迁移，杂交和测序表明，该双峰对应于一个海洋亚硝化单胞菌群，该群最初在由相同沉淀物样品但使用不同PCR引物制备的16S rDNA克隆文库中观察到。我们的数据表明，该新亚硝基亚群被选择用于受污染的鱼类养殖场沉积物中，并且该群的相对丰度受到污染程度的影响。

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