• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied and Environmental Microbiology >Community Structure of Ammonia-Oxidizing Bacteria within Anoxic Marine Sediments
【24h】

Community Structure of Ammonia-Oxidizing Bacteria within Anoxic Marine Sediments

机译：缺氧海洋沉积物中氨氧化细菌的群落结构

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The potential for oxidation of ammonia in anoxic marine sediments exists through anaerobic oxidation by Nitrosomonas-like organisms, utilizing nitrogen dioxide, coupling of nitrification, manganese reduction, and anaerobic oxidation of ammonium by planctomycetes (the Anammox process). Here we describe the presence of microbial communities with the potential to carry out these processes in a natural marine sediment system (Loch Duich, Scotland). Natural microbial communities of Planctomycetales-Verrucomicrobia and β- and γ-proteobacterial ammonia-oxidizing bacteria were characterized by analysis of 16S rRNA genes amplified using group-specific primers by PCR- and reverse transcription-PCR amplification of 16S rDNA and RNA, respectively. Amplification products were analyzed by sequencing of clones and by denaturant gradient gel electrophoresis (DGGE). Amplification of primers specific for Planctomycetales-Verrucomicrobia and β-proteobacterial ammonia-oxidizing bacteria generated products at all sampling sites and depths, but no product was generated using primers specific for γ-proteobacterial ammonia-oxidizing bacteria. 16S rDNA DGGE banding patterns indicated complex communities of β-proteobacterial ammonia-oxidizing bacteria in anoxic marine sediments. Phylogenetic analysis of sequences from clones and those excised from DGGE gels suggests dominance of Nitrosospira cluster 1-like organisms and of strains belonging to a novel cluster represented in dominant bands in 16S rRNA DGGE banding patterns. Their presence indicates a group of organisms closely related to recognized β-proteobacterial ammonia-oxidizing bacteria that may be selected in anoxic environments and may be capable of anoxic ammonia oxidation. Sequence analysis of planctomycete clone libraries and sequences excised from DGGE gels also demonstrated a diverse microbial community and suggested the presence of new subdivisions, but no sequence related to recognized Anammox organisms was detected.
机译：厌氧性类海洋生物通过厌氧氧化，利用二氧化氮，硝化作用，锰还原和浮游菌对铵的厌氧氧化而存在氧化性海洋沉积物中氨的潜力（厌氧氨氧化法） 。在这里，我们描述了微生物群落的存在，这些微生物群落有可能在天然海洋沉积物系统中（苏格兰洛奇·杜伊奇）进行这些过程。通过使用16S rRNA基因组特异性引物对16S进行PCR和逆转录PCR扩增，分析16S rRNA基因，从而表征了 Planctomycetales-Verrucomicrobia 和β-和γ-变形杆菌氨氧化细菌的天然微生物群落。 rDNA和RNA。通过克隆测序和变性梯度凝胶电泳（DGGE）分析扩增产物。对 Planctomycetales-Verrucomicrobia 和β-变形杆菌氨氧化细菌特异的引物的扩增在所有采样位点和深度均产生产物，但是使用对γ-变形细菌氨氧化细菌特异的引物没有生成产物。 16S rDNA DGGE谱带图谱表明缺氧海洋沉积物中复杂的β-变形细菌氨氧化细菌群落。从克隆序列和从DGGE凝胶中切除的序列的系统进化分析表明， Nitrosospira 簇1类生物体占优势，并且属于以16S rRNA DGGE显性谱带的显性谱带代表的新型簇的菌株。它们的存在表明一组与公认的β-变形杆菌氨氧化细菌密切相关的生物，可以在缺氧环境中进行选择，并且能够进行缺氧氨氧化。浮游菌克隆文库的序列分析和从DGGE凝胶中切除的序列还显示了不同的微生物群落，并暗示存在新的细分，但未检测到与公认的Anammox生物有关的序列。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号