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首页> 外文期刊>Applied and Environmental Microbiology >Horizontal Transfer of phnAc Dioxygenase Genes within One of Two Phenotypically and Genotypically Distinctive Naphthalene-Degrading Guilds from Adjacent Soil Environments
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Horizontal Transfer of phnAc Dioxygenase Genes within One of Two Phenotypically and Genotypically Distinctive Naphthalene-Degrading Guilds from Adjacent Soil Environments

机译：来自邻近土壤环境的两个表型和基因型独特的降解萘行会之一中的phnAc双加氧酶基因的水平转移

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Several distinct naphthalene dioxygenases have been characterized to date, which provides the opportunity to investigate the ecological significance, relative distribution, and transmission modes of the different analogs. In this study, we showed that a group of naphthalene-degrading isolates from a polycyclic aromatic hydrocarbon (PAH)-contaminated hillside soil were phenotypically and genotypically distinct from naphthalene-degrading organisms isolated from adjacent, more highly contaminated seep sediments. Mineralization of 14C-labeled naphthalene by soil slurries suggested that the in situ seep community was more acclimated to PAHs than was the in situ hillside community. phnAc-like genes were present in diverse naphthalene-degrading isolates cultured from the hillside soil, while nahAc-like genes were found only among isolates cultured from the seep sediments. The presence of a highly conserved nahAc allele among gram-negative isolates from the coal tar-contaminated seep area provided evidence for in situ horizontal gene transfer and was reported previously (J. B. Herrick, K. G. Stuart-Keil, W. C. Ghiorse, and E. L. Madsen, Appl. Environ. Microbiol. 63:2330-2337, 1997). Natural horizontal transfer of the phnAc sequence was also suggested by a comparison of the phnAc and 16S ribosomal DNA sequences of the hillside isolates. Analysis of metabolites produced by cell suspensions and patterns of amplicons produced by PCR analysis suggested both genetic and metabolic diversity among the naphthalene-degrading isolates of the contaminated hillside. These results provide new insights into the distribution, diversity, and transfer of phnAc alleles and increase our understanding of the acclimation of microbial communities to pollutants.
机译：迄今为止，已经表征了几种不同的萘二加氧酶，这为研究不同类似物的生态意义，相对分布和传播方式提供了机会。在这项研究中，我们表明，从多环芳烃（PAH）污染的山坡土壤中降解萘的分离物在表型和基因型上不同于从相邻的，污染程度更高的渗透性沉积物中分离出的萘降解生物。土壤泥浆对14C标记的萘进行矿化表明，与现场山坡群落相比，原位渗流群落更适合PAHs。在从山坡土壤培养的各种降解萘的菌株中存在phnAc样基因，而仅在从渗流沉积物培养的菌株中发现了nahAc样基因。煤焦油污染的渗漏区的革兰氏阴性分离株中高度保守的nahAc等位基因的存在为原位水平基因转移提供了证据，并且先前已有报道（JB Herrick，KG Stuart-Keil，WC Ghiorse和EL Madsen，Appl环境微生物学》 63：2330-2337，1997）。通过对山坡分离株的phnAc和16S核糖体DNA序列的比较也暗示了phnAc序列的自然水平转移。通过细胞悬浮液产生的代谢产物和通过PCR分析产生的扩增子模式的分析表明，在受污染的山坡上降解萘的萘分离物中，遗传和代谢多样性均存在。这些结果为phnAc等位基因的分布，多样性和转移提供了新的见识，并加深了我们对微生物群落适应污染物的理解。

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