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首页> 外文期刊>FEMS Microbiology Ecology >Enhanced degradation of phenoxyacetic acid in soil by horizontal transfer of the tfdA gene encoding a 2,4-dichlorophenoxyacetic acid dioxygenase
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Enhanced degradation of phenoxyacetic acid in soil by horizontal transfer of the tfdA gene encoding a 2,4-dichlorophenoxyacetic acid dioxygenase

机译：通过水平转移编码2,4-二氯苯氧乙酸双加氧酶的tfdA基因，可增强土壤中苯氧乙酸的降解

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Few studies have investigated the possible impact of in situ gene transfer on the degradation of xenobioric compounds in natural environments. In this work we showed that horizontal transfer of the tfdA gene, carried on plasmid pRO 103, to phenol degrading recipient strains significantly increased the degradation rate of phenoxpacetic acid in sterile and non-sterile soil microcosms. The tfdA gene encodes a 2,4-dichlorophenoxyacetic acid/2-oxoglutarate dioxygenase and by complementation with the phenol degradation pathway an expanded catabolic substrate range, now including phenoxyacetic acid, is evolved. Presence of selective pressure had a positive effect on the emergence of transconjugants. However, even in the absence of phenoxyacetic acid transconjugant populations were detected and were kept at a constant level throughout the experimental period. The residuesphere (interface between decaying plant material and soil matrix) of dry leaves of bailey was shown to bu a hot-spot for gene transfer and presence of barley straw increased the conjugation frequencies in soil microcosms to the same extent as presence of organic nutrients. The results of this study indicate that dissemination of catabolic plasmids is a possible mechanism of genetic adaptation to degradation of xenobiotic compounds in natural environments, and that complementation of catabolic pathways possibly plays an important role in the evolution of new degradative capabilities. The application of horizontal gene transfer as a possible tool in bioremediation of contaminated sites is discussed. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. [References: 31]
机译：很少有研究调查在自然环境中原位基因转移对异生物化合物降解的可能影响。在这项工作中，我们表明，将质粒pRO 103上携带的tfdA基因水平转移到降解苯酚的受体菌株中，可显着提高无菌和非无菌土壤微观世界中苯氧茶酸的降解率。 tfdA基因编码2，4-二氯苯氧基乙酸/ 2-氧代戊二酸双加氧酶，并通过与苯酚降解途径的互补作用，产生了扩展的分解代谢底物范围，现在包括苯氧乙酸。选择压力的存在对转导结合剂的出现具有积极作用。然而，即使在不存在苯氧乙酸的情况下，也能检测到共轭人口，并在整个实验期间保持恒定水平。百利枯叶的残留叶（腐烂植物材料与土壤基质之间的界面）显示出一个热点，可以进行基因转移，大麦秸秆的存在增加了土壤微观世界中与有机养分存在的共轭频率。这项研究的结果表明，分解代谢质粒的传播是遗传适应异源生物化合物在自然环境中降解的可能机制，而分解代谢途径的补充可能在新的降解能力的进化中发挥重要作用。讨论了水平基因转移作为污染部位生物修复中可能的工具的应用。 （C）2001年欧洲微生物学会联合会。由Elsevier Science B.V.保留所有权利。 [参考：31]

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