• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied and Environmental Microbiology >The Enterococcal Surface Protein, Esp, Is Involved in Enterococcus faecalis Biofilm Formation
【24h】

The Enterococcal Surface Protein, Esp, Is Involved in Enterococcus faecalis Biofilm Formation

机译：肠球菌表面蛋白Esp参与粪肠球菌生物膜形成

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The enterococcal surface protein, Esp, is a high-molecular-weight surface protein of unknown function whose frequency is significantly increased among infection-derived Enterococcus faecalisisolates. In this work, a global structural similarity was found between Bap, a biofilm-associated protein of Staphylococcus aureus, and Esp. Analysis of the relationship between the presence of the Esp-encoding gene (esp) and the biofilm formation capacity in E. faecalis demonstrated that the presence of the esp gene is highly associated (P < 0.0001) with the capacity of E. faecalis to form a biofilm on a polystyrene surface, since 93.5% of the E. faecalis esp-positive isolates were capable of forming a biofilm. Moreover, none of the E. faecalis esp-deficient isolates were biofilm producers. Depending on theE. faecalis isolate, insertional mutagenesis ofesp caused either a complete loss of the biofilm formation phenotype or no apparent phenotypic defect. Complementation studies revealed that Esp expression in an E. faecalis esp-deficient strain promoted primary attachment and biofilm formation on polystyrene and polyvinyl chloride plastic from urine collection bags. Together, these results demonstrate that (i) biofilm formation capacity is widespread among clinical E. faecalis isolates, (ii) the biofilm formation capacity is restricted to the E. faecalis strains harboringesp, and (iii) Esp promotes primary attachment and biofilm formation of E. faecalis on abiotic surfaces.
机译：肠球菌表面蛋白Esp是一种功能未知的高分子表面蛋白，其频率在源自感染的粪肠球菌分离株中显着增加。在这项工作中，发现了金黄色葡萄球菌的生物膜相关蛋白Bap与Esp之间的总体结构相似性。分析Esp编码基因（ esp ）的存在与 E中生物膜形成能力之间的关系。粪便证明 esp 基因的存在与 E的能力高度相关（ P <0.0001）。粪便在聚苯乙烯表面形成生物膜，因为 E占93.5％。粪便特别是阳性的分离物能够形成生物膜。而且，没有一个 E。粪便特别是缺乏粪便的分离物是生物膜生产者。取决于 E。粪便分离， esp 的插入诱变导致生物膜形成表型完全丧失或没有明显的表型缺陷。补体研究表明，Em在 E中表达。粪便特别是缺乏粪便的菌株促进了尿液收集袋中聚苯乙烯和聚氯乙烯塑料上的主要附着和生物膜形成。在一起，这些结果表明（i）生物膜形成能力在临床E中广泛存在。粪便分离物，（ii）生物膜形成能力仅限于 E。带有 esp 的粪便菌株，以及（iii）Esp促进 E的初级附着和生物膜形成。粪便在非生物表面上。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号