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首页> 外文期刊>Applied and Environmental Microbiology >Characterization of Recurrent and Sporadic Listeria monocytogenes Isolates from Raw Milk and Nondairy Foods by Pulsed-Field Gel Electrophoresis, Monocin Typing, Plasmid Profiling, and Cadmium and Antibiotic Resistance Determination
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Characterization of Recurrent and Sporadic Listeria monocytogenes Isolates from Raw Milk and Nondairy Foods by Pulsed-Field Gel Electrophoresis, Monocin Typing, Plasmid Profiling, and Cadmium and Antibiotic Resistance Determination

机译：通过脉冲场凝胶电泳，Monocin分型，质粒分析以及镉和抗生素抗性测定来表征生奶和非乳制品中反复出现的零星李斯特菌

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Following previous surveys to assess the incidence ofListeria monocytogenes in raw milk and nondairy foods processed in Northern Ireland, isolates were characterized as recurrent or sporadic on the basis of multilocus enzyme electrophoresis (MEE) analysis and restriction fragment length polymorphism typing. In the present study, 45 representative recurrent and sporadic electrophoretic types (ETs) previously identified by MEE were subjected to pulsed-field gel electrophoresis (PFGE) of genomic DNA macrorestriction fragments, monocin typing, plasmid profiling, and an examination of resistance to cadmium and nine different antibiotics. Although PFGE proved to be capable of subdividing a number of recurrent and sporadic ETs, the grouping of strains arrived at by PFGE and MEE were in broad agreement, and previous conclusions regarding the designation of L. monocytogenes strains as recurrent or sporadic remained unaltered. It is considered that PFGE was able to detect minor genetic changes in recurrent ETs which occurred during the time period in which food surveys were carried out. Production of type E monocin (Types A to E were found among the 45 strains), plasmid carriage, and resistance to cadmium occurred more frequently in recurrent than in sporadic strains and may be important with regard to the ability of L. monocytogenes to persist in food and food-processing environments. Only 2 of 45 strains showed resistance to any of the nine antibiotics tested: two sporadic strains were resistant to tetracycline (MIC, 64 μg ml?1).
机译：经过先前的调查以评估在北爱尔兰加工的生乳和非乳制品中单核细胞增生李斯特菌的发生率，根据多基因座酶电泳（MEE）分析和限制性片段长度多态性分型，将分离株表征为复发性或散发性。在本研究中，对先前由MEE鉴定出的45种代表性的复发性和散发性电泳类型（ET）进行了基因组DNA宏限制性片段的脉冲场凝胶电泳（PFGE），一元蛋白分型，质粒分析以及对镉和镉的抗性检查九种不同的抗生素。尽管PFGE被证明能够细分许多复发性和散发性ET，但由PFGE和MEE得出的菌株的分类是一致的，并且先前关于将单核细胞增生李斯特氏菌命名为复发性或散发性菌株的结论仍然没有改变。据认为，PFGE能够检测到在进行食物调查的这段时间内发生的复发性ET中的微小遗传变化。与散发性菌株相比，E型单抗的生产（在45个菌株中发现A型至E型），质粒运输和对镉的抗药性比散发性菌株更频繁地发生，这可能对单核细胞增生李斯特菌的持久性至关重要。食品和食品加工环境。 45个菌株中只有2个对所测试的9种抗生素具有抗药性：2个散发菌株对四环素具有抗药性（MIC，64μgml？1）。

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