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首页> 外文期刊>Applied and Environmental Microbiology >Chromosomal and Symbiotic Relationships of Rhizobia Nodulating Medicago truncatula and M. laciniata
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Chromosomal and Symbiotic Relationships of Rhizobia Nodulating Medicago truncatula and M. laciniata

机译：根瘤菌结实苜蓿和紫花苜蓿的染色体与共生关系

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Multilocus sequence typing (MLST) is a sequence-based method used to characterize bacterial genomes. This method was used to examine the genetic structure of Medicago-nodulating rhizobia at the Amra site, which is located in an arid region of Tunisia. Here the annual medics Medicago laciniata and M. truncatula are part of the natural flora. The goal of this study was to identify whether distinct chromosomal groups of rhizobia nodulate M. laciniata because of its restricted requirement for specific rhizobia. The MLST analysis involved determination of sequence variation in 10 chromosomal loci of 74 isolates each of M. laciniata and M. truncatula. M. truncatula was used as a control trap host, because unlike M. laciniata, it has relatively unrestricted rhizobial requirements. Allelic diversity among the plasmid nodC alleles in the isolates was also determined. The 148 isolates were placed into 26 chromosomal sequence types (STs), only 3 of which had been identified previously. The rhizobia of M. laciniata were shown to be part of the general Medicago-nodulating population in the soil because 99.95% of the isolates had chromosomal genotypes similar to those recovered from M. truncatula. However, the isolates recovered from M. laciniata were less diverse than those recovered from M. truncatula, and they also harbored an unusual nodC allele. This could perhaps be best explained by horizontal transfer of the different nodC alleles among members of the Medicago-nodulating rhizobial population at the field site. Evidence indicating a history of lateral transfer of rhizobial symbiotic genes across distinct chromosomal backgrounds is provided.
机译：多基因座序列分型（MLST）是一种用于表征细菌基因组的基于序列的方法。该方法用于检查位于突尼斯干旱地区的Amra地点的紫花苜蓿根瘤菌的遗传结构。这里一年生的军医Medicago laciniata和M. truncatula是天然菌群的一部分。这项研究的目的是确定由于根瘤菌对特定根瘤菌的需求有限，根瘤菌的不同染色体组是否会结节。 MLST分析涉及确定74个分离株（M. laciniata和M. truncatula）的10个染色体基因座中的序列变异。 truncatula分枝杆菌被用作控制陷阱宿主，因为与lactisata lactisata不同，它具有相对不受限制的根瘤菌要求。还确定了分离物中质粒nodC等位基因之间的等位基因多样性。将148个分离株放入26种染色体序列类型（ST）中，其中只有3种先前已被鉴定。由于99.95％的分离株的染色体基因型与从截枝分枝杆菌中回收的相似，因此，M。laciniata的根瘤菌是土壤中普通紫花苜蓿的一部分。然而，从乳杆菌中分离出的分离株与从截短杆菌中分离出的分离株差异较小，并且它们还具有不寻常的nodC等位基因。也许可以通过在田间站点上的有紫花病菌根瘤菌种群中不同nodC等位基因的水平转移来最好地解释这一点。提供了表明横纹共生基因在不同染色体背景中横向转移的历史的证据。

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