• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied and Environmental Microbiology >Signature-Tagged Mutagenesis of Edwardsiella ictaluri Identifies Virulence-Related Genes, Including a Salmonella Pathogenicity Island 2 Class of Type III Secretion Systems
【24h】

Signature-Tagged Mutagenesis of Edwardsiella ictaluri Identifies Virulence-Related Genes, Including a Salmonella Pathogenicity Island 2 Class of Type III Secretion Systems

机译：爱德华氏菌的签名标记诱变鉴定与毒力有关的基因，包括沙门氏菌致病岛2类III型分泌系统。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Edwardsiella ictaluri is the leading cause of mortality in channel catfish culture, but little is known about its pathogenesis. The use of signature-tagged mutagenesis in a waterborne infection model resulted in the identification of 50 mutants that were unable to infect/survive in catfish. Nineteen had minitransposon insertions in miscellaneous genes in the chromosome, 10 were in genes that matched to hypothetical proteins, and 13 were in genes that had no significant matches in the NCBI databases. Eight insertions were in genes encoding proteins associated with virulence in other pathogens, including three in genes involved in lipopolysaccharide biosynthesis, three in genes involved in type III secretion systems (TTSS), and two in genes involved in urease activity. With the use of a sequence from a lambda clone carrying several TTSS genes, Blastn analysis of the partially completed E. ictaluri genome identified a 26,135-bp pathogenicity island containing 33 genes of a TTSS with similarity to the Salmonella pathogenicity island 2 class of TTSS. The characterization of a TTSS apparatus mutant indicated that it retained its ability to invade catfish cell lines and macrophages but was defective in intracellular replication. The mutant also invaded catfish tissues in numbers equal to those of invading wild-type E. ictaluri bacteria but replicated poorly and was slowly cleared from the tissues, while the wild type increased in number.
机译：爱德华氏菌是channel鱼养殖中死亡的主要原因，但对其发病机理知之甚少。在水传播的感染模型中使用标记标签的诱变技术导致鉴定了50个无法在cat鱼中感染/存活的突变体。在染色体的其他基因中有19个具有小转座子插入，在与假设蛋白质匹配的基因中有10个在NCBI数据库中没有显着匹配的基因中有13个。在编码与其他病原体毒性相关的蛋白质的基因中有八次插入，包括与脂多糖生物合成有关的基因中的三处，与III型分泌系统（TTSS）有关的基因中的三处和与尿素酶活性有关的基因中的三处。利用带有几个TTSS基因的lambda克隆中的序列，对部分完成的E.ictaluri基因组进行Blastn分析，确定了一个26,135 bp的致病岛，其中包含33种TTSS基因，与TTSS沙门氏菌致病岛2类相似。 TTSS装置突变体的表征表明，它保留了入侵cat鱼细胞系和巨噬细胞的能力，但在细胞内复制方面存在缺陷。该突变体还入侵cat鱼组织，其数量与入侵野生型大肠杆菌的数量相同，但复制较差，并从组织中缓慢清除，而野生型却增加。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号