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首页> 外文期刊>Applied and Environmental Microbiology >Colonization of Cattle Intestines by Campylobacter jejuni and Campylobacter lanienae
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Colonization of Cattle Intestines by Campylobacter jejuni and Campylobacter lanienae

机译：空肠弯曲杆菌和兰氏弯曲杆菌对牛肠的定殖

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The location and abundance of Campylobacter jejuni and Campylobacter lanienae in the intestines of beef cattle were investigated using real-time quantitative PCR in two studies. In an initial study, digesta and tissue samples were obtained along the digestive tract of two beef steers known to shed C. jejuni and C. lanienae (steers A and B). At the time of slaughter, steer B weighed 540 kg, compared to 600 kg for steer A, yet the intestine of steer B (40.5 m) was 36% longer than the intestine of steer A (26.1 m). In total, 323 digesta samples (20-cm intervals) and 998 tissue samples (3.3- to 6.7-cm intervals) were processed. Campylobacter DNA was detected in the digesta and in association with tissues throughout the small and large intestines of both animals. Although C. jejuni and C. lanienae DNA were detected in both animals, only steer A contained substantial quantities of C. jejuni DNA. In both digesta and tissues of steer A, C. jejuni was present in the duodenum and jejunum. Considerable quantities of C. jejuni DNA also were observed in the digesta obtained from the cecum and ascending colon, but minimal DNA was associated with tissues of these regions. In contrast, steer B contained substantial quantities of C. lanienae DNA, and DNA of this bacterium was limited to the large intestine (i.e., the cecum, proximal ascending colon, descending colon, and rectum); the majority of tissue-associated C. lanienae DNA was present in the cecum, descending colon, and rectum. In a second study, the location and abundance of C. jejuni and C. lanienae DNA were confirmed in the intestines of 20 arbitrarily selected beef cattle. DNA of C. jejuni and C. lanienae were detected in the digesta of 57% and 95% of the animals, respectively. C. jejuni associated with intestinal tissues was most abundant in the duodenum, ileum, and rectum. However, one animal contributed disproportionately to the abundance of C. jejuni DNA in the ileum and rectum. C. lanienae was most abundant in the large intestine, and the highest density of DNA of this bacterium was found in the cecum. Therefore, C. jejuni colonized the proximal small intestine of asymptomatic beef cattle, whereas C. lanienae primarily resided in the cecum, descending colon, and rectum. This information could be instrumental in developing efficacious strategies to manage the release of these bacteria from the gastrointestinal tracts of cattle.
机译：在两项研究中，使用实时定量PCR研究了空肠弯曲杆菌和兰氏弯曲杆菌在肉牛肠道中的位置和丰度。在最初的研究中，沿两条已知排空空肠弯曲杆菌和兰氏弯曲杆菌的牛肉公牛（公牛A和牛公牛B）的消化道采集了消化物和组织样本。宰杀时，ste牛B重540公斤，而ste牛A重600公斤，但B牛B的肠（40.5 m）比A牛A的肠（26.1 m）长36％。总共处理了323个消化物样本（间隔20厘米）和998个组织样本（间隔3.3到6.7厘米）。在两只动物的小肠和大肠的消化物中以及与组织相关的地方都检测到弯曲杆菌DNA。尽管在两只动物中均检测到空肠弯曲杆菌和兰氏弯曲杆菌DNA，但仅only牛A含有大量空肠弯曲杆菌DNA。在转向A的消化和组织中，空肠弯曲杆菌均存在于十二指肠和空肠中。在从盲肠和升结肠获得的消化物中还观察到了相当数量的空肠弯曲杆菌DNA，但是与这些区域的组织相关的DNA最少。相反，转向B包含大量的兰氏梭菌DNA，并且该细菌的DNA限于大肠（即盲肠，近端升结肠，降结肠和直肠）。大部分与组织相关的兰氏梭菌DNA存在于盲肠，降结肠和直肠中。在第二项研究中，在20头任意选择的肉牛的肠中确认了空肠弯曲杆菌和兰氏弯曲杆菌DNA的位置和丰度。在动物的消化物中分别检测到空肠弯曲杆菌和兰氏弯曲杆菌的DNA，分别为57％和95％。与肠组织相关的空肠弯曲杆菌在十二指肠，回肠和直肠中含量最高。然而，一只动物对回肠和直肠中空肠弯曲杆菌DNA的丰富程度起着不成比例的作用。兰色梭菌在大肠中含量最高，盲肠中发现该细菌的DNA密度最高。因此，空肠弯曲杆菌定殖在无症状肉牛的近端小肠中，而兰氏弯曲杆菌主要生活在盲肠，降结肠和直肠中。这些信息可能有助于制定有效的策略来管理这些细菌从牛胃肠道的释放。

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