• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Rapid Quantitative Microbiological Assay of Antibiotics and Chemical Preservatives of a Nonantibiotic Nature
【24h】

Rapid Quantitative Microbiological Assay of Antibiotics and Chemical Preservatives of a Nonantibiotic Nature

机译：抗生素和非抗生素性质的化学防腐剂的快速定量微生物测定

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A rapid quantitative assay of 14 antibiotics and 6 chemical preservatives by the cylinder-plate diffusion method is described, in which Bacillus stearothermophilus ATCC 12980 was used as the test organism. After 4 hr of incubation at 65 C, the zones of inhibition can be read off easily with the naked eye. The 4-hr assay was compared under identical conditions with the conventional 16- to 18-hr agar diffusion assay, i.e., by using the test organisms and media proposed by the Code of Federal Regulations, and no difference in accuracy between the two methods was detected. In both cases, the coefficient of variation for replicate tests was less than 2%. The test strain is highly sensitive to penicillins, cephalosporins, and bacitracin and is consequently also particularly suitable for the determination of low-level concentrations of these substances in aqueous solutions and in urine.
机译：描述了一种通过圆柱板扩散法对14种抗生素和6种化学防腐剂进行快速定量测定的方法，其中使用嗜热脂肪芽孢杆菌ATCC 12980作为测试生物。在65°C下孵育4小时后，可以用肉眼轻松读出抑制区域。在相同的条件下，将4小时的测定与常规的16至18小时的琼脂扩散测定进行了比较，即通过使用《联邦法规》提议的受试生物和培养基，两种方法的准确度没有差异检测到。在这两种情况下，重复测试的变异系数均小于2％。该测试菌株对青霉素，头孢菌素和杆菌肽高度敏感，因此也特别适用于测定水溶液和尿液中这些物质的低浓度。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号