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Multi-Biochemical Test System for Distinguishing Enteric and Other Gram-Negative Bacilli

机译：区分肠杆菌和其他革兰氏阴性杆菌的多生化测试系统

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A multi-biochemical test system consisting of nine tests, entitled Enterotube, was evaluated in parallel with conventional tests to determine its value in the identification of enteric and certain other gram-negative bacilli. The 242 bacterial strains studied were from a variety of pathological specimens and from our culture collection. When the results with individual tests represented in both test systems were compared, no discrepancies were noted in the indole test, and one discrepancy was recorded for dextrose. In 7 of 242 hydrogen sulfide tests, 3 of 242 phenylalanine tests, 22 of 242 urease tests, 15 of 242 dulcitol tests, 12 of 242 lactose tests, 27 of 217 lysine decarboxylase tests, and 5 of 242 citrate tests, the Enterotube results were contrary to those obtained with conventional methods. The lysine decarboxylase test in the Enterotube posed a problem of interpretation and readability and is not an acceptable alternative to the conventional methods. Fifteen of the strains studied were incorrectly identified by the Enterotube system and four could not be differentiated from other closely related strains. Salmonella could be identified as to group, whereas Shigella strains were frequently misidentified as Escherichia. The Enterotube method is simple and convenient, and all media are inoculated at once from a single colony.
机译：与常规测试同时评估了一个由9个测试组成的多重生化测试系统，称为Enterotube，以确定其在鉴定肠杆菌和某些其他革兰氏阴性杆菌中的价值。研究的242个细菌菌株来自各种病理标本和我们的培养物收集。当比较两个测试系统中代表的单个测试的结果时，吲哚测试中没有发现差异，并且葡萄糖的记录有一个差异。在242个硫化氢测试中的7个，242个苯丙氨酸测试中的3个，242个脲酶测试中的22个，242个二甘醇测试中的15个，242个乳糖测试中的12个，217个赖氨酸脱羧酶测试中的27个和242个柠檬酸盐测试中的5个，肠小肠的结果是与常规方法获得的结果相反。肠管中的赖氨酸脱羧酶测试存在解释和可读性的问题，不是常规方法的可接受替代方法。所研究的菌株中有15个被Enterotube系统错误地识别，其中4个无法与其他密切相关的菌株区分开。可将沙门氏菌归为一组，而志贺氏菌菌株经常被误认为埃希氏菌。 Enterotube方法简便易行，所有培养基都可以从单个菌落中一次接种。

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