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Simplified, Accurate Method for Antibiotic Assay of Clinical Specimens

机译：简化，准确的临床标本抗生素检测方法

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Large glass plates are used for this modified agar-well diffusion assay method, allowing up to 81 replications on a single plate. With a specially designed agar punch, it is possible to prepare the small agar wells very quickly. The saving in serum resulting from fewer replications of standards with the large plates, and the small volume of the agar wells, makes it economically feasible to use pooled human serum for the standard antibiotic solutions. Methods are described for preparing the standard solutions, and for providing controls for the deterioration of standards and unknowns. Procedures for preparing and maintaining the commonly used assay organisms are presented. Serum specimens are tested directly rather than diluting them to a narrow range of antibiotic concentrations. This is possible because of a procedure for calculations that recognizes the curvilinear relationship between zone sizes and antibiotic concentrations. Adaptation of this method to a number of the commonly used antibiotics is described. With this method, it has been possible to test large numbers of clinical specimens in a minimal time, and with accuracy consistently better than 10%.
机译：大型玻璃板用于此改良的琼脂井扩散分析方法，在单个板上最多可重复81次。使用专门设计的琼脂打孔器，可以非常快速地准备小琼脂孔。由于标准板在大板中的复制较少以及琼脂孔的体积较小，因此节省了血清，这使得将合并的人血清用于标准抗生素溶液在经济上是可行的。描述了用于制备标准溶液以及为标准和未知物的降解提供控制的方法。介绍了制备和维护常用测定生物的程序。血清标本应直接测试，而不是稀释至窄范围的抗生素浓度。这是可能的，因为可以识别区域大小和抗生素浓度之间的曲线关系的计算过程。描述了该方法对许多常用抗生素的适应性。使用这种方法，可以在最短的时间内测试大量的临床标本，且精度始终优于10％。

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