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Enzymatic Techniques for the Study of Pathways of Carbohydrate Utilization in the Rumen

机译：瘤胃糖利用途径的酶学研究

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Enzymatic techniques were used to study the metabolism of carbohydrates by ruminal bacteria. A direct relationship was observed between the proportions of acetate and propionate formed and the specific activities of the enzymes which participate in forming these acids. An inverse relationship between butyrate formation and butyrate-forming enzymes was observed. The relative activities of succinic dehydrogenase to fumaric reductase, nicotinamide adenine dinucleotide-linked glutamic dehydrogenase to nicotinamide adenine dinucleotide phosphate-linked glutamic dehydrogenase, and pyridine nucleotide-nonlinked lactic dehydrogenase to pyridine nucleotide-linked lactic dehydrogenase were affected by the level of concentrates in the diet. Lactyl coenzyme A dehydrase activity was below the limits of the assay technique in many samples from the alfalfa hay diet, and increased to relatively high levels when concentrates were fed. It is suggested that the enzymatic method will prove valuable for studying the contributions of individual microorganisms to the overall ruminal metabolism, and, with certain limitations, useful for estimating the relative contributions of alternate pathways.
机译：酶促技术被用于研究瘤胃细菌对碳水化合物的代谢。在形成的乙酸盐和丙酸酯的比例与参与形成这些酸的酶的比活性之间观察到直接的关系。观察到丁酸酯形成与丁酸酯形成酶之间的反比关系。琥珀酸脱氢酶对富马酸还原酶的相对活性，烟酰胺腺嘌呤二核苷酸连接的谷氨酸脱氢酶与烟酰胺腺嘌呤二核苷酸磷酸连接的谷氨酸脱氢酶，吡啶核苷酸非连接乳酸脱氢酶对吡啶核苷酸连接的乳酸脱氢酶的相对活性。饮食。在苜蓿干草饮食的许多样品中，乳酸辅酶A的脱水酶活性均低于测定技术的极限，饲喂浓缩物时，乳酸酶的脱水酶活性降低至相对较高的水平。建议该酶方法对于研究单个微生物对整个瘤胃代谢的贡献将是有价值的，并且在一定的限制下，可用于估算替代途径的相对贡献。

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