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Determination of Drug Resistance of Mycobacterium tuberculosis Cultures

机译：结核分枝杆菌培养物耐药性的测定

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A total of 3,303 strains of Mycobacterium tuberculosis were tested for sensitivity to streptomycin (SM), isoniazid (INH), and p-aminosalicylic acid (PAS) by the Steenken modified minimal inhibitory concentration (MIC) test. A simultaneous double blind comparison was carried out on 277 selected strains by the Steenken MIC test and the Canetti proportion method. Agreement between the results for the two tests was 82% for SM, 95% for INH, and 89% for PAS. A small number of strains appeared to be sensitive when tested by one method but resistant by the other. MIC determinations were carried out on 83 strains by using Steenken-Smith, Lowenstein-Jensen, and Middlebrook 7H10 media containing a more extended range of concentrations of the test drugs. The MIC values for both SM and dihydrostreptomycin increased on Steenken-Smith medium compared with the other two. INH did not show any medium effect, whereas PAS showed increased MIC values in 7H10 agar. The significance of the comparisons of the MIC values on the various media is discussed in terms of possible changes in the drug sensitivity testing methods used at present in this laboratory.
机译：通过Steenken改良最小抑菌浓度（MIC）测试，共测试了3,303株结核分枝杆菌对链霉素（SM），异烟肼（INH）和对氨基水杨酸（PAS）的敏感性。通过Steenken MIC测试和Canetti比例法对277个选定的菌株进行了同时双盲比较。两次测试结果之间的一致性为SM为82％，INH为95％，PAS为89％。当通过一种方法测试时，少数菌株似乎是敏感的，但通过另一种方法可以抵抗。使用含有更广泛浓度的测试药物的Steenken-Smith，Lowenstein-Jensen和Middlebrook 7H10培养基对83个菌株进行MIC测定。与其他两种相比，在Steenken-Smith培养基上SM和二氢链霉素的MIC值均增加。 INH未显示任何中等作用，而PAS在7H10琼脂中显示MIC值增加。根据该实验室目前使用的药物敏感性测试方法的可能变化，讨论了在各种介质上比较MIC值的重要性。

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