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首页> 外文期刊>Applied Microbiology >Potential Pathogens in the Environment: Cultural Reactions and Nucleic Acid Studies on Klebsiella pneumoniae from Clinical and Environmental Sources
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Potential Pathogens in the Environment: Cultural Reactions and Nucleic Acid Studies on Klebsiella pneumoniae from Clinical and Environmental Sources

机译：环境中的潜在病原体：临床和环境来源的肺炎克雷伯菌的培养反应和核酸研究

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The phenotypic and nucleic acid properties of Klebsiella pneumoniae have been studied on cultures obtained from six different habitats (humans, vegetables, seeds, trees, rivers, and pulp mills). The 19 cultural reactions of 107 isolates varied significantly only in tryptophanase activity and dulcitol fermentation. The percentage of guanine plus cytosine base composition of 41 isolates varied from 53.9 to 59.2%. The range of percentage of guanine plus cytosine base composition for environmental klebsiellas was broader than that for the cultures of human origin. The range of deoxyribonucleic acid relative reassociation (homology) to the human K. pneumoniae reference strain extended from 5% to 100% and the chromosome molecular weights ranged from 2,200 × 106 to 3,000 × 106. The species of K. pneumoniae is thus molecularly more heterogeneous than previously thought and most isolates of human, pulp mill, and river origin are genetically indistinguishable. The presence of K. pneumoniae therefore represents a deterioration of the microbiological quality of the environment and should be considered of public health significance. At the present time the health significance of the molecularly more divergent strains, primarily of vegetable and seed origin, their relationship to klebsiellas of human origin, or to other genera of the Enterobacteriaceae is unclear.
机译：肺炎克雷伯菌的表型和核酸特性已经在从六个不同的生境（人类，蔬菜，种子，树木，河流和制浆厂）获得的培养物中进行了研究。 107个分离株的19种培养反应仅在色氨酸酶活性和二糖醇发酵方面有显着差异。 41个分离株的鸟嘌呤加胞嘧啶碱基组成的百分比在53.9％至59.2％之间。环境克雷伯菌的鸟嘌呤加胞嘧啶碱基组成的百分比范围比人类来源的培养物的范围宽。脱氧核糖核酸与人肺炎克雷伯氏菌参考菌株的相对重组（同源性）范围从5％扩展到100％，染色体分子量范围从2,200×106到3,000×106。因此，肺炎克雷伯菌的种类为在分子上比以前认为的异质，人类，纸浆厂和河流起源的大多数分离物在遗传上是无法区分的。因此，肺炎克雷伯菌的存在代表了环境微生物质量的下降，应被认为具有公共卫生意义。目前，尚不清楚主要是植物和种子来源的分子差异更大的菌株，它们与人类起源的克雷伯菌或与肠杆菌科其他属的关系对健康的重要性。

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