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Novel High-Throughput Detection Method To Assess Bacterial Surfactant Production

机译：评估细菌表面活性剂产量的新型高通量检测方法

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A novel biosurfactant detection assay was developed for the observation of surfactants on agar plates. By using an airbrush to apply a fine mist of oil droplets, surfactants can be observed instantaneously as halos around biosurfactant-producing colonies. This atomized oil assay can detect a wide range of different synthetic and bacterially produced surfactants. This method could detect much lower concentrations of many surfactants than a commonly used water drop collapse method. It is semiquantitative and therefore has broad applicability for uses such as high-throughput mutagenesis screens of biosurfactant-producing bacterial strains. The atomized oil assay was used to screen for mutants of the plant pathogen Pseudomonas syringae pv. syringae B728a that were altered in the production of biosurfactants. Transposon mutants displaying significantly altered surfactant halos were identified and further analyzed. All mutants identified displayed altered swarming motility, as would be expected of surfactant mutants. Additionally, measurements of the transcription of the syringafactin biosynthetic cluster in the mutants, the principal biosurfactant known to be produced by B728a, revealed novel regulators of this pathway.
机译：开发了一种新型的生物表面活性剂检测方法，用于观察琼脂平板上的表面活性剂。通过使用喷枪施加细微的油滴雾，可以立即观察到表面活性剂产生生物活性剂菌落周围的光环。这种雾化油测定法可以检测多种不同的合成和细菌产生的表面活性剂。与常用的水滴崩解方法相比，该方法可以检测到许多表面活性剂浓度低得多的溶液。它是半定量的，因此对于诸如生产生物表面活性剂的细菌菌株的高通量诱变筛选等用途具有广泛的适用性。雾化油分析用于筛选植物病原体丁香假单胞菌pv的突变体。生物表面活性剂生产中发生变化的丁香丁香B728a。转座子突变体显示出明显改变的表面活性剂晕被鉴定并进一步分析。如表面活性剂突变体所预期的那样，所有鉴定出的突变体均显示出群运动的改变。另外，对突变体中丁香脂素生物合成簇的转录的测量表明已知该途径是新型的调节剂，该突变体是已知由B728a产生的主要生物表面活性剂。

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