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首页> 外文期刊>Applied Microbiology >Survival of T3 Coliphage in Varied Extracellular Environments. I. Viability of the Coliphage During Storage and in Aerosols
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Survival of T3 Coliphage in Varied Extracellular Environments. I. Viability of the Coliphage During Storage and in Aerosols

机译：T3鹅毛在各种细胞外环境中的存活。一，储存和气溶胶中鹅粪的生存力

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摘要

The objective of this study was to determine the feasibility of using airborne T3 coliphage as a viral tracer in microbial aerosols. Although T3 coliphage was relatively stable when stored either at temperatures ranging from 21 to 37 C or in the frozen state at -20 C, there was a 2-log loss in infectivity when stored for 72 days at 4 C. Either agitation of stored coliphage suspensions held at 31 C or wide fluctuations in storage temperature produced an increased loss of infectivity. In the airborne state, freshly prepared coliphage and stored coliphage behaved similarly, with survival diminishing as the relative humidity (RH) was lowered. The greatest loss occurred during the first five min following aerosolization. The results showed that only under certain conditions of temperature and relative humidity can T3 coliphage be used as a satisfactory aerosol tracer.
机译：这项研究的目的是确定在微生物气溶胶中使用机载T3噬菌体作为病毒示踪剂的可行性。尽管T3噬菌体在21至37°C的温度下或在-20°C的冷冻状态下贮存时相对稳定，但在4°C下贮存72天时，感染力损失了2个对数。保持在31°C的悬浮液或储存温度的大幅波动会增加传染性的损失。在空气传播状态下，新鲜制备的大肠杆菌和储存的噬菌体表现相似，存活率随着相对湿度（RH）的降低而降低。最大的损失发生在雾化后的前五分钟内。结果表明，只有在一定温度和相对湿度条件下，T3噬菌体才能用作令人满意的气溶胶示踪剂。

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