• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Physiological Studies of an Oligosporogenous Strain of Bacillus popilliae
【24h】

Physiological Studies of an Oligosporogenous Strain of Bacillus popilliae

机译：一株小芽孢杆菌的少孢菌的生理研究

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A relatively small but consistent increase in the frequency of spore formation by an oligosporogenous strain of Bacillus popilliae (NRRL B-2309M) was obtained by adding 0.1% sodium pyruvate to the sporulation medium. The frequency of spore formation was essentially the same when a low level of glucose, trehalose, or glucose-6-phosphate or a high level of α-methyl-d-mannoside was added as the carbon and energy source. Many other variations in the cultural medium and cultural conditions failed to enhance spore formation of 2309M, and no spores were found in four asporogenic strains under any of the conditions tried. There were no significant differences between the 2309M strain and three nonsporeforming cultures with respect to (i) the rate and extent of growth, (ii) the rates of glucose utilization, or (iii) volatile acid production and utilization. None of the cultures tested was found to produce detectable levels of extracellular protease or an antibiotic. The only consistent marker found associated with spore formation was the development of catalase activity, and this activity was stimulated by heating at 80 C for 10 min. This was not found unless morphological evidence of spore formation was observed. The germination of the spores formed by 2309M in vitro was stimulated by heat shock and by the addition of pyruvate to the germination medium.
机译：通过向孢子形成培养基中添加0.1％的丙酮酸钠，可以使由寡孢子的芽孢杆菌菌株（NRRL B-2309M）产生的孢子形成频率相对较小但始终如一。当添加低水平的葡萄糖，海藻糖或葡萄糖-6-磷酸或高水平的α-甲基-d-甘露糖苷作为碳和能源时，孢子形成的频率基本相同。培养基和培养条件的许多其他变化均未能增强2309M的孢子形成，并且在任何尝试的条件下，均未在4种产孢菌株中发现孢子。 2309M菌株与三种非孢子形成培养物在（i）生长速率和程度，（ii）葡萄糖利用速率或（iii）挥发性酸的产生和利用方面无显着差异。没有发现所测试的培养物产生可检测水平的细胞外蛋白酶或抗生素。发现与孢子形成有关的唯一一致的标记是过氧化氢酶活性的发展，并且通过在80℃加热10分钟来刺激该活性。除非观察到孢子形成的形态学证据，否则未发现。通过热激和向萌发培养基中添加丙酮酸盐刺激2309M体外形成的孢子的萌发。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号