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Accuracy and Sensitivity of Commercial PCR-Based Methods for Detection of Salmonella enterica in Feed

机译：基于商业PCR的饲料中沙门氏菌检测方法的准确性和敏感性

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The present study compared the performance of commercial PCR-based Salmonella enterica detection methods (BAX System Q7, the iQ-Check Salmonella II kit, and the TaqMan Salmonella enterica detection kit) with culture-based methods (modified semisolid Rappaport-Vassiliadis [MSRV] and NMKL71) in spiked and naturally contaminated samples of feed mill scrapings (FMS), palm kernel meal (PKM), pelleted feed (PF), rape seed meal (RSM), soybean meal (SM), and wheat grain (WG). When results from the various feeds were compared, the number of Salmonella enterica CFU/25 g required to produce a positive were as follows: PKM > FMS = WG > RSM = SM = PF. These data are similar to those developed in earlier studies with culture-based Salmonella detection methods. PCR-based methods were performed similarly to culture-based methods, with respect to sensitivity and specificity. However, many PCR positives could not be confirmed by Salmonella isolation and for that reason the evaluated methods were found to be suitable only when rapid results were paramount. Nevertheless, PCR-based methods cannot presently replace culture-based methods when typing information is required for tracing studies or epidemiological investigations. The observed difference in detection levels is a potential problem when prevalence data are compared as well as when feed ingredients are tested for conformance with microbiological criteria. This paper also presents a statistical model that describes the detection probability when different levels (CFU) of Salmonella contamination are present in feed materials.
机译：本研究比较了基于商业PCR的沙门氏菌检测方法（BAX System Q7，iQ-Check 沙门氏菌 II试剂盒和TaqMan 沙门氏菌）的性能。 检测试剂盒），采用基于培养的方法（改良的半固体Rappaport-Vassiliadis [MSRV]和NMKL71）在加料和自然污染的饲料厂碎料（FMS），棕榈仁粕（PKM），颗粒饲料（PF）中取样，油菜籽粉（RSM），大豆粉（SM）和小麦籽粒（WG）。当比较各种饲料的结果时，产生阳性结果所需的肠沙门氏菌 CFU / 25 g的数量如下：PKM> FMS = WG> RSM = SM = PF。这些数据与早期研究中基于培养物的沙门氏菌检测方法获得的数据相似。在敏感性和特异性方面，基于PCR的方法与基于培养的方法相似。然而，沙门氏菌的分离并不能证实许多PCR阳性，因此，只有在快速获得结果至关重要的情况下，所评估的方法才是合适的。但是，当追踪研究或流行病学调查需要输入类型信息时，基于PCR的方法目前不能替代基于文化的方法。当比较患病率数据以及测试饲料成分是否符合微生物标准时，观察到的检测水平差异是一个潜在的问题。本文还提供了一个统计模型，该模型描述了当饲料原料中存在不同浓度的沙门氏菌污染时的检测概率。

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