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Radiation Sterilization of Prototype Military Foods. III. Pork Loin

机译:原型军用食品的辐射灭菌。三,猪里脊

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Ten lots of pork loin, packed in cans, were inoculated with approximately 106Clostridium botulinum spores per can. Each lot was seeded with a different strain; five type A and five type B strains were used. The pack comprised 5,690 cans, including controls, and contained about 109 spores per dose. The cans were irradiated with Co60 in the range of 0 to 5.0 Mrad (0.5 Mrad increments) at 5 to 25 C, incubated for 6 months at 30 C, and examined for swelling, toxicity, and recoverable C. botulinum. The minimal experimental sterilizing dose (ESD) based on nonswollen, nontoxic, but nonsterile end points was 2.5 D equivalent, varied with the method of computation: 4.74, 4.33 ± 0.17, and 4.19 to 4.99 Mrad were obtained by the Weibull, Spearman-Bárber, and Schmidt-Nank techniques, respectively. Calculation of D and MRD values by the conventional Schmidt-Nank method produced increasing values with rising dosage; this finding was compared with the data derived by the other two methods of calculation. Suggestions for estimating the MRD of a prototype radiation process are offered.
机译:每罐装十罐猪里脊肉,每罐约接种106支肉毒梭状芽孢。每一批都接种了不同的菌株。使用了五个A型和五个B型菌株。该包装包括5,690罐,包括对照,每剂量约含109个孢子。在5至25℃下用0至5.0兆拉德(增量为0.5兆拉德)的Co 60照射罐,在30℃下孵育6个月,并检查其溶胀,毒性和可回收的肉毒梭菌。基于非溶胀,无毒,但非无菌终点的最小实验灭菌剂量(ESD)为2.5 D当量,随计算方法的不同而变化:Weibull,Spearman-Bárber获得了4.74、4.33±0.17和4.19至4.99 Mrad ,和Schmidt-Nank技术。用常规的Schmidt-Nank方法计算D和MRD值会随着剂量的增加而增加。将该发现与其他两种计算方法得出的数据进行了比较。提供估计原型辐射过程的MRD的建议。

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