Plaque Assay for Pneumonia Virus of Mice

Gerard Shimonaski; Paul E. Came

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Plaque Assay for Pneumonia Virus of Mice

机译：小鼠肺炎病毒的噬斑测定

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A plaque assay for the quantitation of pneumonia virus of mice is described. To obtain reproducible plaque formation, proteolytic enzymes had to be incorporated in the overlay medium. The plaque morphology observed in the presence of pancreatin and chymotrypsin was superior to that seen with trypsin. Although the plaque assay was found to be slightly less sensitive than the TCID50 determination described by Harter and Choppin, it enables cloning of the virus by plaque selection and permits further study of pneumonia virus of mice.

机译：描述了用于定量小鼠肺炎病毒的噬斑测定。为了获得可重现的噬斑形成，必须将蛋白水解酶掺入覆盖培养基中。在胰酶和胰凝乳蛋白酶存在下观察到的噬菌斑形态优于胰蛋白酶。尽管发现噬斑测定的灵敏度比Harter和Choppin所描述的TCID50测定稍低，但它可以通过噬菌斑选择克隆病毒，并可以进一步研究小鼠的肺炎病毒。

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《Applied Microbiology》 |1970年第5期|共3页
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Gerard Shimonaski; Paul E. Came;
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专利

1. Virus-replicating T cells in the immune response of mice. I. Virus plaque assay of the lymphocytes reactive to sheep erythrocytes. [J] . N Minato, Y Katsura The Journal of Experomental Medicine . 1977,第2期

机译：病毒复制性T细胞在小鼠的免疫反应中。 I.对绵羊红细胞有反应性的淋巴细胞的病毒噬斑测定。
2. Evaluation of a Plaque Assay for the Maedi-Progressive Pneumonia-Visna Viruses [J] . R. S. Trowbridge Applied Microbiology . 1974,第3期

机译：Maedi-渐进性肺炎-Visna病毒的噬斑分析评估
3. Comparison of the hemagglutination inhibition procedure and an enzyme-linked immunosorbent assay for detection of specific antibodies to pneumonia virus of mice in experimentally infected laboratory rats. [J] . J P Desc?teaux, P Payment, M Trudel Journal of Clinical Microbiology . 1980,第2期

机译：血凝抑制程序和酶联免疫吸附测定法的比较，用于检测实验感染的实验鼠的小鼠抗肺炎病毒的特异性抗体。
4. Effect of Mycoplasma hyopneumoniae vaccination on clinical parameters and economic performance of pigs co-infected with M. Hyopneumoniae and Porcine Circovirus Type 2 [C] . Rapp-Gabrielson, V., Sornsen, Proceedings of the 3rd Congress of the Asian Pig Veterinary Society . 2007

机译：猪肺炎支原体疫苗接种对猪肺炎支原体和2型猪圆环病毒共同感染猪的临床参数和经济性能的影响
5. Fatal bacteremia is observed in mice co-infected with filamentous influenza A virus and serotype 3 Streptococcus pneumoniae. [D] . Speshock, Janice L. 2007

机译：在感染了丝状A型流感病毒和3型肺炎链球菌的小鼠中观察到致命菌血症。
6. Plaque Assay for Pneumonia Virus of Mice [O] . Gerard Shimonaski, Paul E. Came 1970

机译：小鼠肺炎病毒的噬斑测定
7. Plaque Assay for Pneumonia Virus of Mice [O] . Gerard Shimonaski, Paul E. Came 1970

机译：用于小鼠肺炎病毒的斑块测定
8. Detection of Environmental Viruses in Sludge: Enhancement of Enterovirus Plaque Assay Titers with 5-Iodo-2'-Deoxyuridine and Comparison to Adenovirus and Coliphage Titers (Journal Version) [R] . Williams, F. P. , Hurst, C. J. 1988

机译：污泥中环境病毒的检测：用5-Iodo-2'-Deoxyuridine增强肠道病毒噬斑测定滴度并与腺病毒和大肠杆菌滴度进行比较（期刊版）

Plaque Assay for Pneumonia Virus of Mice

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