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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of the hemagglutination inhibition procedure and an enzyme-linked immunosorbent assay for detection of specific antibodies to pneumonia virus of mice in experimentally infected laboratory rats.
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Comparison of the hemagglutination inhibition procedure and an enzyme-linked immunosorbent assay for detection of specific antibodies to pneumonia virus of mice in experimentally infected laboratory rats.

机译：血凝抑制程序和酶联免疫吸附测定法的比较，用于检测实验感染的实验鼠的小鼠抗肺炎病毒的特异性抗体。

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摘要

An enzyme-linked immunosorbent assay (ELISA) and the hemagglutination inhibition (HI) test were used to evaluate the response of laboratory rats to experimental infection with pneumonia virus of mice. The ELISA procedure was more sensitive than the HI test and detected very low levels of antibodies early in the course of infection. At 5 days postinfection, ELISA detected antibody increases in five of five animals, whereas only two of five increases were detected by the HI test. At 9 days postinfection, the HI test failed to detect one titer increase measured by ELISA. Later during the course of infection, increases were detected by both tests. The ELISA procedure was, in general, more sensitive for detecting low levels of antibody than was the HI test, but was equal in sensitivity when high titers of antibody were measured.
机译：酶联免疫吸附试验（ELISA）和血凝抑制试验（HI）用于评估实验大鼠对实验性小鼠肺炎病毒感染的反应。 ELISA程序比HI测试更灵敏，并且在感染过程中早期检测到非常低的抗体水平。感染后5天，ELISA检测到五只动物中有五只增加了抗体，而HI测试只检测到五只增加了两只。感染后9天，HI测试未能检测到通过ELISA测定的1滴度增加。在感染过程的后期，两个测试均检测到增加。通常，ELISA方法对于检测低水平的抗体比HI测试更敏感，但在测量高滴度的抗体时，其灵敏度相同。

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