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Pyrolysis-Gas-Liquid Chromatography of Fungi: Differentiation of Species and Strains of Several Members of the Aspergillus flavus Group

机译:真菌的热解-气相色谱:黄曲霉菌群几个成员的种类和菌株的区分

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Four fungi of the Aspergillus flavus group were differentiated to the species level and strain level by pyrolysis-gas-liquid chromotography. Comparisons of pyrochromatograms revealed more similarities than dissimilarities among both species and strains in the pyrolytic elution patterns. Quantitative analysis was made by comparing the number of peaks in which two strains or reference species agreed or disagreed, the degree of superimposability between the pyrolytic elution patterns of strains and reference species, and the presence or absence of peaks for strain pairs within each species. The accuracy and precision of these techniques suggest that pyrolysis-gas-liquid chromatography may have wide application in the detection, enumeration, and identification of fungi by nonmycologically trained personnel.
机译:通过热解-气-液色谱法将黄曲霉组的四种真菌区分为菌种和菌种。热解色谱图的比较显示,在热解洗脱模式中,物种和菌株之间的相似性大于差异。通过比较两个菌株或参比物种一致或不同意的峰数,菌株与参比物种的热解洗脱模式之间的叠加程度以及每个物种中菌株对的存在或不存在峰,进行定量分析。这些技术的准确性和精密度表明,热解气液色谱法可能在未经真菌学培训的人员检测,计数和鉴定真菌方面具有广泛的应用。

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