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首页> 外文期刊>Applied Microbiology >Development of a DNA Microarray for Enterococcal Species, Virulence, and Antibiotic Resistance Gene Determinations among Isolates from Poultry
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Development of a DNA Microarray for Enterococcal Species, Virulence, and Antibiotic Resistance Gene Determinations among Isolates from Poultry

机译：家禽分离物中肠球菌物种，毒力和抗生素抗性基因测定的DNA芯片开发。

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A DNA microarray (Enteroarray) was designed with probes targeting four species-specific taxonomic identifiers to discriminate among 18 different enterococcal species, while other probes were designed to identify 18 virulence factors and 174 antibiotic resistance genes. In total, 262 genes were utilized for rapid species identification of enterococcal isolates, while characterizing their virulence potential through the simultaneous identification of endogenous antibiotic resistance and virulence genes. Enterococcal isolates from broiler chicken farms were initially identified by using the API 20 Strep system, and the results were compared to those obtained with the taxonomic genes atpA , recA , pheS , and ddl represented on our microarray. Among the 171 isolates studied, five different enterococcal species were identified by using the API 20 Strep system: Enterococcus faecium , E. faecalis , E. durans , E. gallinarum , and E. avium . The Enteroarray detected the same species as API 20 Strep, as well as two more: E. casseliflavus and E. hirae . Species comparisons resulted in 15% (27 isolates) disagreement between the two methods among the five API 20 Strep identifiable species and 24% (42 isolates) disagreement when considering the seven Enteroarray identified species. The species specificity of key antibiotic and virulence genes identified by the Enteroarray were consistent with the literature adding further robustness to the redundant taxonomic probe data. Sequencing of the cpn60 gene further confirmed the complete accuracy of the microarray results. The new Enteroarray should prove to be a useful tool to accurately genotype strains of enterococci and assess their virulence potential.
机译：设计了一种DNA微阵列（Enteroarray），其探针针对四种物种特异性的生物分类学标识符，以区分18种不同的肠球菌物种，而其他探针旨在鉴定18种毒力因子和174种抗生素抗性基因。总共有262个基因被用于快速鉴定肠球菌菌株，同时通过同时鉴定内源抗生素抗性和毒力基因来表征其毒力潜力。最初使用API​​ 20 Strep系统鉴定了来自肉鸡场的肠球菌，并将其结果与通过我们的微阵列代表的分类基因atpA，recA，pheS和ddl获得的结果进行了比较。通过使用API​​ 20 Strep系统，在研究的171个分离株中鉴定出了五种不同的肠球菌：粪肠球菌，粪肠球菌，杜兰肠球菌，鸡肠球菌和鸟肠球菌。 Enteroarray检测到与API 20链球菌相同的物种，另外还有两个：casseliflavus和hirae。物种比较导致在5种API 20链球菌可鉴定物种中两种方法之间存在15％（27个分离株）的分歧，而考虑到七个由Enteroarray鉴定的物种时，则有24％（42个分离株）的分歧。 Enteroarray鉴定的关键抗生素和毒力基因的物种特异性与文献一致，为冗余分类探针数据增加了进一步的稳健性。 cpn60基因的测序进一步证实了微阵列结果的完全准确性。新的Enteroarray应该被证明是准确鉴定肠球菌基因型菌株并评估其毒力潜力的有用工具。

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