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首页> 外文期刊>Applied Microbiology >Food as a Source for Quorum Sensing Inhibitors: Iberin from Horseradish Revealed as a Quorum Sensing Inhibitor of Pseudomonas aeruginosa
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Food as a Source for Quorum Sensing Inhibitors: Iberin from Horseradish Revealed as a Quorum Sensing Inhibitor of Pseudomonas aeruginosa

机译：食物作为群体感应抑制剂的来源：辣根中的伊比林被揭示为铜绿假单胞菌的群体感应抑制剂

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Foods with health-promoting effects beyond nutritional values have been gaining increasing research focus in recent years, although not much has been published on this subject in relation to bacterial infections. With respect to treatment, a novel antimicrobial strategy, which is expected to transcend problems with selective pressures for antibiotic resistance, is to interrupt bacterial communication, also known as quorum sensing (QS), by means of signal antagonists, the so-called QS inhibitors (QSIs). Furthermore, QSI agents offer a potential solution to the deficiencies associated with use of traditional antibiotics to treat infections caused by bacterial biofilms and multidrug-resistant bacteria. Several QSIs of natural origin have been identified, and in this study, several common food products and plants were extracted and screened for QSI activity in an attempt to isolate and characterize previously unknown QSI compounds active against the common opportunistic pathogen Pseudomonas aeruginosa . Several extracts displayed activity, but horseradish exhibited the highest activity. Chromatographic separation led to the isolation of a potent QSI compound that was identified by liquid chromatography-diode array detector-mass spectrometry (LC-DAD-MS) and nuclear magnetic resonance (NMR) spectroscopy as iberin—an isothiocyanate produced by many members of the Brassicaceae family. Real-time PCR (RT-PCR) and DNA microarray studies showed that iberin specifically blocks expression of QS-regulated genes in P. aeruginosa .
机译：近年来，具有营养促进作用而不是营养价值的食品已成为越来越多的研究焦点，尽管有关细菌感染的这一问题尚未发表。关于治疗，一种新的抗微生物策略有望通过信号拮抗剂（即所谓的QS抑制剂）中断细菌传播，也称为群体感应（QS），从而克服抗生素耐药的选择性压力问题。 （QSI）。此外，QSI试剂为解决与使用传统抗生素治疗由细菌生物膜和耐多药细菌引起的感染相关的缺陷提供了一种潜在的解决方案。已经鉴定出几种天然来源的QSI，在这项研究中，提取了几种常见的食品和植物并筛选了QSI活性，以试图分离和鉴定对常见的机会性病原体铜绿假单胞菌具有活性的QSI化合物。几种提取物表现出活性，但是辣根表现出最高的活性。色谱分离导致了强效QSI化合物的分离，该化合物通过液相色谱-二极管阵列检测器质谱（LC-DAD-MS）和核磁共振（NMR）光谱鉴定为艾伯林-一种由许多成员生产的异硫氰酸酯。十字花科。实时PCR（RT-PCR）和DNA芯片研究表明，iberin特异性阻断QS调控的基因在铜绿假单胞菌中的表达。

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