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首页> 外文期刊>Applied Microbiology >Pressure Transducer Method for Measuring Gas Production by Microorganisms
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Pressure Transducer Method for Measuring Gas Production by Microorganisms

机译：测量微生物产气的压力传感器方法

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A simple method for measuring gas production by microorganisms by using a pressure transducer to sense pressure buildup was developed and tested with members of the coliform group. The test system consisted of a 5.0 lb/in2 pressure transducer and a pressure equalizer valve attached to the metal cap of a test tube (20 by 150 mm); gas pressure was recorded on a strip-chart recorder. Gas pressure response curves consisted of (i) a lag period with no marked increase in pressure, (ii) a rapid pressure buildup period, and (iii) a leveling-off period. A linear relationship was established between inoculum size and length of the lag period. Cultures shaken at 200 oscillations/min showed a marked increase in rate of gas release over stationary cultures. Cell concentrations at the time of rapid buildup in pressure were 108/ml. Mean maximum pressure recordings, lb/in2 per 10 ml of broth, were: Enterobacter aerogenes, 3.70; Citrobacter intermedium, 2.70; and Escherichia coli, 2.10. Mean CO2 concentrations, ppm of headspace gas, for E. coli were: (i) 2,000 at time of inoculation, (ii) 25,000 at time of rapid buildup in pressure, and (iii) 150,000 at maximum pressure. These results indicate the potential application of the pressure transducer method for rapidly detecting coliforms and other gas-producing microorganisms in clinical samples and in sterility testing of foods.
机译：开发了一种简单的方法，该方法通过使用压力传感器感应压力积累来测量微生物产生的气体，并与大肠菌群的成员一起进行了测试。测试系统由一个5.0 lb / in2的压力传感器和一个安装在试管金属帽（20 x 150毫米）上的均压阀组成。气体压力记录在带状图记录仪上。气压响应曲线由（i）压力没有明显升高的滞后期，（ii）压力快速建立期和（iii）稳定期组成。接种量与滞后期的长度之间建立了线性关系。与固定培养物相比，以200次振荡/分钟摇动的培养物显示出气体释放速率的显着增加。压力迅速升高时的细胞浓度为108 / ml。平均最大压力记录，每10毫升肉汤1b / in2，为：产气肠杆菌3.70;中间柠檬酸杆菌2.70;大肠杆菌2.10。大肠杆菌的平均CO2浓度（顶空气体ppm）为：（i）接种时为2,000，（ii）压力迅速升高时为25,000，和（iii）最大压力时为150,000。这些结果表明压力传感器方法在快速检测临床样品中的大肠菌和其他产气微生物以及食品无菌测试中的潜在应用。

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