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首页> 外文期刊>Applied Microbiology >Environmental Factors Affect Acidobacterial Communities below the Subgroup Level in Grassland and Forest Soils
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Environmental Factors Affect Acidobacterial Communities below the Subgroup Level in Grassland and Forest Soils

机译：环境因素影响草地和森林土壤中亚组水平以下的酸性细菌群落

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In soil, Acidobacteria constitute on average 20% of all bacteria, are highly diverse, and are physiologically active in situ . However, their individual functions and interactions with higher taxa in soil are still unknown. Here, potential effects of land use, soil properties, plant diversity, and soil nanofauna on acidobacterial community composition were studied by cultivation-independent methods in grassland and forest soils from three different regions in Germany. The analysis of 16S rRNA gene clone libraries representing all studied soils revealed that grassland soils were dominated by subgroup Gp6 and forest soils by subgroup Gp1 Acidobacteria . The analysis of a large number of sites ( n = 57) by 16S rRNA gene fingerprinting methods (terminal restriction fragment length polymorphism [T-RFLP] and denaturing gradient gel electrophoresis [DGGE]) showed that Acidobacteria diversities differed between grassland and forest soils but also among the three different regions. Edaphic properties, such as pH, organic carbon, total nitrogen, C/N ratio, phosphorus, nitrate, ammonium, soil moisture, soil temperature, and soil respiration, had an impact on community composition as assessed by fingerprinting. However, interrelations with environmental parameters among subgroup terminal restriction fragments (T-RFs) differed significantly, e.g., different Gp1 T-RFs correlated positively or negatively with nitrogen content. Novel significant correlations of Acidobacteria subpopulations (i.e., individual populations within subgroups) with soil nanofauna and vascular plant diversity were revealed only by analysis of clone sequences. Thus, for detecting novel interrelations of environmental parameters with Acidobacteria , individual populations within subgroups have to be considered.
机译：在土壤中，酸性细菌平均构成所有细菌的20％，具有高度的多样性，并具有原位生理活性。但是，它们的个别功能以及与土壤中较高分类单元的相互作用仍然未知。在这里，通过耕种独立方法研究了德国三个不同地区的草地和森林土壤中土地利用，土壤特性，植物多样性和土壤纳米动物对酸性细菌群落组成的潜在影响。对代表所有研究土壤的16S rRNA基因克隆文库的分析表明，草地土壤以Gp6亚群为主，森林土壤以Gp1酸杆菌亚群为主。通过16S rRNA基因指纹图谱分析方法（末端限制性片段长度多态性[T-RFLP]和变性梯度凝胶电泳[DGGE]）对大量位点（n = 57）进行分析，结果表明，草地和森林土壤的酸性细菌多样性有所不同，但在三个不同地区之间如指纹图谱所示，pH，有机碳，总氮，碳氮比，磷，硝酸盐，铵，土壤湿度，土壤温度和土壤呼吸等深层特性对群落组成都有影响。但是，亚组末端限制性片段（T-RF）之间与环境参数的相互关系存在显着差异，例如，不同的Gp1 T-RF与氮含量呈正相关或负相关。仅通过克隆序列分析揭示了酸性细菌亚群（即亚组内的个体种群）与土壤纳米动物和维管植物多样性的新型显着相关性。因此，为了检测新的环境参数与嗜酸菌的相互关系，必须考虑亚组内的各个种群。

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