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Intracellular Free Iron and Its Potential Role in Ultrahigh-Pressure-Induced Inactivation of Escherichia coli

机译:细胞内游离铁及其在超高压诱导的大肠杆菌失活中的潜在作用

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摘要

Intracellular free iron of Escherichia coli was determined by whole-cell electron paramagnetic resonance spectrometry. Ultrahigh pressure (UHP) increased both intracellular free iron and cell lethality in a pressure-dose-dependent manner. The iron chelator 2,2′-dipyridyl protected cells against UHP treatments. A mutation that produced iron overload conditions sensitized E. coli to UHP treatment.
机译:通过全细胞电子顺磁共振光谱法测定大肠杆菌的细胞内游离铁。超高压(UHP)以压力剂量依赖性方式增加细胞内游离铁和细胞致死率。铁螯合剂2,2'-联吡啶保护细胞免受UHP处理。产生铁超负荷条件的突变使大肠杆菌对UHP处理敏感。

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