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首页> 外文期刊>Applied Microbiology >Real-Time Solvent Tolerance Analysis of Pseudomonas sp. Strain VLB120ΔC Catalytic Biofilms
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Real-Time Solvent Tolerance Analysis of Pseudomonas sp. Strain VLB120ΔC Catalytic Biofilms

机译：假单胞菌sp。实时溶剂耐受性分析。菌株VLB120ΔC催化生物膜

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Biofilms are ubiquitous surface-associated microbial communities embedded in an extracellular polymeric (EPS) matrix, which gives the biofilm structural integrity and strength. It is often reported that biofilm-grown cells exhibit enhanced tolerance toward adverse environmental stress conditions, and thus there has been a growing interest in recent years to use biofilms for biotechnological applications. We present a time- and locus-resolved, noninvasive, quantitative approach to study biofilm development and its response to the toxic solvent styrene. Pseudomonas sp. strain VLB120ΔC-BT- gfp 1 was grown in modified flow-cell reactors and exposed to the solvent styrene. Biofilm-grown cells displayed stable catalytic activity, producing ( S )-styrene oxide continuously during the experimental period. The pillar-like structure and growth rate of the biofilm was not influenced by the presence of the solvent. However, the cells experience severe membrane damage during styrene treatment, although they obviously are able to adapt to the solvent, as the amount of permeabilized cells decreased from 75 to 80% down to 40% in 48 h. Concomitantly, the fraction of concanavalin A (ConA)-stainable EPS increased, substantiating the assumption that those polysaccharides play a major role in structural integrity and enhanced biofilm tolerance toward toxic environments. Compared to control experiments with planktonic grown cells, the Pseudomonas biofilm adapted much better to toxic concentrations of styrene, as nearly 65% of biofilm cells were not permeabilized (viable), compared to only 7% in analogous planktonic cultures. These findings underline the robustness of biofilms under stress conditions and its potential for fine chemical syntheses.
机译：生物膜是嵌入到细胞外聚合物（EPS）基质中的无处不在的与表面相关的微生物群落，可提供生物膜的结构完整性和强度。经常报道生物膜生长的细胞对不利的环境压力条件表现出增强的耐受性，因此近年来对将生物膜用于生物技术应用的兴趣日益增长。我们提出了一种时间和地点解析的，非侵入性的定量方法来研究生物膜的发展及其对有毒溶剂苯乙烯的响应。假单胞菌菌株VLB120ΔC-BT-gfp1在改良的流通池反应器中生长，并暴露于溶剂苯乙烯中。生物膜生长的细胞表现出稳定的催化活性，在实验期间连续产生（S）-环氧乙烷。生物膜的柱状结构和生长速率不受溶剂存在的影响。然而，这些细胞在苯乙烯处理过程中会遭受严重的膜破坏，尽管它们显然能够适应溶剂，因为在48小时内，可渗透细胞的数量从75％降至80％降至40％。同时，伴刀豆球蛋白A（ConA）染色的EPS的比例增加，证实了这些多糖在结构完整性和增强的生物膜对毒性环境的耐受性中起主要作用的假设。与浮游性生长细胞的对照实验相比，假单胞菌生物膜对苯乙烯的毒性浓度适应性更好，因为近65％的生物膜细胞没有被渗透（存活），而在类似的浮游培养中只有7％。这些发现强调了生物膜在压力条件下的坚固性及其精细化学合成的潜力。

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