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首页> 外文期刊>Applied Microbiology >Cellular Response of the Amoeba Acanthamoeba castellanii to Chlorine, Chlorine Dioxide, and Monochloramine Treatments
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Cellular Response of the Amoeba Acanthamoeba castellanii to Chlorine, Chlorine Dioxide, and Monochloramine Treatments

机译：变形虫阿米巴虫对氯，二氧化氯和一氯胺处理的细胞响应

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摘要

Acanthamoeba castellanii is a free-living amoebae commonly found in water systems. Free-living amoebae might be pathogenic but are also known to bear phagocytosis-resistant bacteria, protecting these bacteria from water treatments. The mode of action of these treatments is poorly understood, particularly on amoebae. It is important to examine the action of these treatments on amoebae in order to improve them. The cellular response to chlorine, chlorine dioxide, and monochloramine was tested on A. castellanii trophozoites. Doses of disinfectants leading to up to a 3-log reduction were compared by flow cytometry and electron microscopy. Chlorine treatment led to size reduction, permeabilization, and retraction of pseudopods. In addition, treatment with chlorine dioxide led to a vacuolization of the cytoplasm. Monochloramine had a dose-dependent effect. At the highest doses monochloramine treatment resulted in almost no changes in cell size and permeability, as shown by flow cytometry, but the cell surface became smooth and dense, as seen by electron microscopy. We show that these disinfectants globally induced size reduction, membrane permeabilization, and morphological modifications but that they have a different mode of action on A. castellanii .
机译：棘阿米巴棘阿米巴（Acanthamoeba castellanii）是一种自由生活的变形虫，通常存在于水系统中。自由活动的变形虫可能是致病的，但也已知具有抗吞噬作用的细菌，可以保护这些细菌免于水处理。这些治疗的作用方式了解甚少，尤其是在变形虫上。重要的是检查这些治疗对变形虫的作用，以改善它们。在卡氏棘孢菌滋养体上测试了细胞对氯，二氧化氯和一氯胺的反应。通过流式细胞仪和电子显微镜比较了导致3-log降低的消毒剂量。氯处理导致假足的尺寸减小，通透化和缩回。另外，用二氧化氯处理导致细胞质的空泡化。一氯胺具有剂量依赖性作用。如流式细胞术所示，在最高剂量下，一氯胺处理几乎不会导致细胞大小和通透性的变化，但如电子显微镜所见，细胞表面变得光滑而致密。我们表明，这些消毒剂可引起尺寸减小，膜透化和形态学改变，但是它们对A. castellanii具有不同的作用方式。

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