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Molecular Detection of Campylobacter spp. in California Gull (Larus californicus) Excreta

机译：弯曲杆菌属的分子检测。在加利福尼亚鸥（Larus californicus）排泄物

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摘要

We examined the prevalence, quantity, and diversity of Campylobacter species in the excreta of 159 California gull ( Larus californicus ) samples using culture-, PCR-, and quantitative PCR (qPCR)-based detection assays. Campylobacter prevalence and abundance were relatively high in the gull excreta examined; however, C. jejuni and C. lari were detected in fewer than 2% of the isolates and DNA extracts from the fecal samples that tested positive. Moreover, molecular and sequencing data indicated that most L. californicus campylobacters were novel (<97% 16S rRNA gene sequence identity to known Campylobacter species) and not closely related to species commonly associated with human illness. Campylobacter estimates were positively related with those of fecal indicators, including a gull fecal marker based on the Catellicoccus marimammalium 16S rRNA gene.
机译：我们使用基于培养，PCR和基于定量PCR（qPCR）的检测方法，检查了159只加利福尼亚海鸥（Larus californicus）的排泄物中弯曲杆菌属的数量，数量和多样性。在所检查的海鸥排泄物中，弯曲杆菌的患病率和丰度相对较高;但是，从粪便样本中检测到阳性的分离株和DNA提取物中，只有不到2％的细菌检测到空肠弯曲杆菌和拉里弯曲杆菌。而且，分子和测序数据表明，大多数加州乳杆菌弯曲杆菌是新颖的（与已知弯曲杆菌属物种的<97％16S rRNA基因序列同一性），与通常与人类疾病相关的物种没有密切关系。弯曲杆菌的估计值与粪便指示物的估计值呈正相关，包括基于粪杯中卡特彼勒球菌16S rRNA基因的鸥粪便标记物。

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