Quantitative Analysis of the Relative Transcript Levels of Four Chlorophenol Reductive Dehalogenase Genes in Desulfitobacterium hafniense PCP-1 Exposed to Chlorophenols

Ariane Bisaillon; Réjean Beaudet; Fran?ois Lépine; Richard Villemur

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Quantitative Analysis of the Relative Transcript Levels of Four Chlorophenol Reductive Dehalogenase Genes in Desulfitobacterium hafniense PCP-1 Exposed to Chlorophenols

机译：暴露于氯酚的哈弗脱硫杆菌PCP-1中四个氯酚还原脱卤酶基因相对转录水平的定量分析

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Relative to those of unexposed cultures, the transcript levels of the four CprA-type reductive dehalogenase genes ( cprA2 , cprA3 , cprA4 , and cprA5 ) in Desulfitobacterium hafniense PCP-1 were measured in cultures exposed to chlorophenols. In 2,4,6-trichlorophenol-amended cultures, cprA2 and cprA3 were upregulated, as was cprA5 , but concomitantly with the appearance of 2,4-dichlorophenol (DCP). In 3,5-DCP-amended cultures, only cprA5 was upregulated. In pentachlorophenol-amended cultures grown for 12 h, cprA2 and cprA3 were upregulated but not cprA5. cprA4 was not upregulated significantly in cultures containing any tested chlorophenols.

机译：相对于未暴露的培养物，在暴露于氯酚的培养物中，检测了Hafniense Hafniense PCP-1中四个CprA型还原性脱卤素酶基因（cprA2，cprA3，cprA4和cprA5）的转录水平。在2,4,6-三氯苯酚修饰的培养物中，cprA2和cprA3和cprA5一样被上调，但同时伴有2,4-二氯苯酚（DCP）的出现。在3,5-DCP修正的培养物中，仅cprA5被上调。在经过五氯苯酚修饰的培养物中培养12小时，cprA2和cprA3被上调，而cprA5没有被上调。在含有任何测试的氯酚的培养物中，cprA4没有明显上调。

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《Applied Microbiology》 |2011年第17期|共4页
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Ariane Bisaillon; Réjean Beaudet; Fran?ois Lépine; Richard Villemur;
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1. Identification and Characterization of a Novel CprA Reductive Dehalogenase Specific to Highly Chlorinated Phenols from Desulfitobacterium hafniense Strain PCP-1 [J] . Ariane Bisaillon, Réjean Beaudet, Fran?ois Lépine, Applied Microbiology . 2010,第22期

机译：鉴定和表征新型的CprA还原脱卤酶，其特异于来自嗜盐脱硫杆菌PCP-1的高度氯化的苯酚。
2. Identification and Characterization of a Novel CprA Reductive Dehalogenase Specific to Highly Chlorinated Phenols from Desulfitobacterium hafniense Strain PCP-1 [J] . Ariane Bisaillon, Réjean Beaudet, Fran?ois Lépine, Applied and Environmental Microbiology . 2010,第22期

机译：鉴定和表征新型的CprA还原脱卤酶，其特异于来自嗜盐脱硫杆菌PCP-1的高度氯化的苯酚。
3. Occurrence of several genes encoding putative reductive dehalogenases in Desulfitobacterium hafniense/frappieri and Dehalococcoides ethenogenes. [J] . Villemur R, Saucier M, Gauthier A, Canadian journal of microbiology . 2002,第8期

机译：在哈弗氏脱硫杆菌/ frappieri和埃德洛球菌中编码推定的还原性脱卤素酶的几种基因的出现。
4. System level SEUs propagation analysis via data flow-based reduction and quantitative model checking [C] . Ghaith Bany Hamad, Otmane Ait Mohamed 2017 First International Conference on Embedded amp; Distributed Systems . 2017

机译：通过基于数据流的缩减和定量模型检查进行系统级SEU传播分析
5. Analysis of multiple levels of vascular-plant phylogeny using a nuclear RNA polymerase subunit gene (RPB2) and a large plastid data set: Studies of the tracheophytes, cycads and the lilies and relatives [D] . Zgurski, Jessie 2004

机译：使用核RNA聚合酶亚基基因（RPB2）和大量质体数据集分析多种水平的维管束系统发育：气管植物，苏铁，百合和亲戚的研究
6. Quantitative Analysis of the Relative Transcript Levels of Four Chlorophenol Reductive Dehalogenase Genes in Desulfitobacterium hafniense PCP-1 Exposed to Chlorophenols [O] . Ariane Bisaillon, Réjean Beaudet, François Lépine, 2011

机译：暴露于氯酚的哈弗脱硫杆菌PCP-1中四个氯酚还原脱卤酶基因的相对转录水平的定量分析
7. Quantitative Analysis of the Relative Transcript Levels of Four Chlorophenol Reductive Dehalogenase Genes in Desulfitobacterium hafniense PCP-1 Exposed to Chlorophenols ▿ [O] . Bisaillon, Ariane, Beaudet, Réjean, Lépine, François, 2011

机译：暴露于氯酚的哈弗脱硫杆菌PCP-1中四个氯酚还原性脱卤酶基因的相对转录水平的定量分析▿

Quantitative Analysis of the Relative Transcript Levels of Four Chlorophenol Reductive Dehalogenase Genes in Desulfitobacterium hafniense PCP-1 Exposed to Chlorophenols

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