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首页> 外文期刊>Applied Microbiology >Resistance of Bacillus subtilis Spores to Inactivation by Gamma Irradiation and Heating in the Presence of a Bactericide III. Factors Affecting Rates of Inactivation by Phenylmercuric Nitrate
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Resistance of Bacillus subtilis Spores to Inactivation by Gamma Irradiation and Heating in the Presence of a Bactericide III. Factors Affecting Rates of Inactivation by Phenylmercuric Nitrate

机译：枯草芽孢杆菌孢子对在有杀菌剂III的情况下通过伽马辐射和加热灭活的抗性。影响硝酸苯汞灭活率的因素

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摘要

Aqueous suspensions of Bacillus subtilis NCTC 8236 spores, surviving gamma irradiation from a cesium-137 source, exhibited an enhanced rate of inactivation compared to nonirradiated spores when heated with 0.04% phenylmercuric nitrate. The enhanced rate of inactivation, observable from survival curves, was noted when spores were irradiated with 150,000 rad under air in either the presence or absence of the bactericide. The magnitude of the enhanced inactivation rate increased as the irradiation dose under air increased from 150,000 to 300,000 rad. The inactivation rates of spores surviving irradiation with 150,000 rad under either oxic or anoxic conditions did not exhibit a simple quantitative relationship. The enhancement effect was observed when the severity of the heat treatment was increased by either reducing the pH from 8 to 6 or raising the temperature from 70 to 90 C.
机译：与0.04％硝酸苯汞加热时，与未辐照的孢子相比，枯草芽孢杆菌NCTC 8236孢子的水悬浮液（从铯137来源进行伽玛射线辐照）仍能存活，其失活率更高。从存活曲线观察到的灭活率提高，是在有或没有杀菌剂的条件下，在空气中用150,000 rad辐射孢子。随着空气中的辐照剂量从150,000 rad增加到300,000 rad，增强的失活率的幅度增加。在有氧或无氧条件下经150,000 rad照射后存活的孢子的失活率均未表现出简单的定量关系。当通过将pH值从8降低到6或将温度从70升高到90 C来提高热处理的强度时，可以观察到增强效果。

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