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Cellulolytic Bacteria Associated with Sloughing Spoilage of California Ripe Olives

机译:纤维素分解细菌与加利福尼亚成熟橄榄变质腐败相关

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Sloughing spoilage of California ripe olives during processing is characterized by severe softening, skin rupture, and flesh sloughing. It was assumed that cellulolytic activity was responsible for skin rupture and sloughing of flesh, and so a deliberate search was made for cellulolytic bacteria from olives undergoing sloughing spoilage. A bacterium identified as Cellulomonas flavigena was highly cellulolytic, attacking filter paper, carboxymethyl cellulose (CMC) gel, and olive tissue. Other bacteria attacking CMC, but not filter paper, enhanced the activity of the Cellulomonas strain when grown in mixed culture, although they did not, in pure culture, have any effect on filter paper. These latter cultures (all degraded olive tissue) represented the genera Xanthomonas, Aerobacter, and Escherichia. Other noncellulolytic bacteria belonging to the genera Alcaligenes, Kurthia, and Micrococcus also were used for study of mixed culture fermentation of cellulose by C. flavigena. Cellobiose accumulation at levels of 1.0% (w/v) and above suppressed growth of C. flavigena.
机译:加工过程中加利福尼亚成熟橄榄的腐烂变质的特征是严重软化,皮肤破裂和果肉腐烂。假定纤维素分解活性是造成皮肤破裂和肉脱落的原因,因此,人们有意寻找来自腐烂腐败橄榄的纤维素分解细菌。被鉴定为黄杆菌纤维杆菌的细菌是高度纤维素分解性的,侵蚀性滤纸,羧甲基纤维素(CMC)凝胶和橄榄组织。当在混合培养中生长时,其他攻击CMC的细菌(而不是滤纸)会增强纤维单胞菌菌株的活性,尽管在纯培养中它们不会对滤纸产生任何影响。后面的这些培养物(所有降解的橄榄组织)代表了Xanthomonas,Aerobacter和Escherichia属。属于Alcaligenes,Kurthia和Micrococcus属的其他非纤维素分解细菌也被用于研究黄曲霉纤维素的混合培养发酵。纤维二糖在1.0%(w / v)或更高水平的积累抑制了黄曲霉的生长。

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