• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Comparative Genomic and Functional Analysis of Lactobacillus casei and Lactobacillus rhamnosus Strains Marketed as Probiotics
【24h】

Comparative Genomic and Functional Analysis of Lactobacillus casei and Lactobacillus rhamnosus Strains Marketed as Probiotics

机译：作为益生菌销售的干酪乳杆菌和鼠李糖乳杆菌菌株的基因组和功能比较分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Four Lactobacillus strains were isolated from marketed probiotic products, including L. rhamnosus strains from Vifit (Friesland Campina) and Idoform (Ferrosan) and L. casei strains from Actimel (Danone) and Yakult (Yakult Honsa Co.). Their genomes and phenotypes were characterized and compared in detail with L. casei strain BL23 and L. rhamnosus strain GG. Phenotypic analysis of the new isolates indicated differences in carbohydrate utilization between L. casei and L. rhamnosus strains, which could be linked to their genotypes. The two isolated L. rhamnosus strains had genomes that were virtually identical to that of L. rhamnosus GG, testifying to their genomic stability and integrity in food products. The L. casei strains showed much greater genomic heterogeneity. Remarkably, all strains contained an intact spaCBA pilus gene cluster. However, only the L. rhamnosus strains produced mucus-binding SpaCBA pili under the conditions tested. Transcription initiation mapping demonstrated that the insertion of an iso -IS 30 element upstream of the pilus gene cluster in L. rhamnosus strains but absent in L. casei strains had constituted a functional promoter driving pilus gene expression. All L. rhamnosus strains triggered an NF-κB response via Toll-like receptor 2 (TLR2) in a reporter cell line, whereas the L. casei strains did not or did so to a much lesser extent. This study demonstrates that the two L. rhamnosus strains isolated from probiotic products are virtually identical to L. rhamnosus GG and further highlights the differences between these and L. casei strains widely marketed as probiotics, in terms of genome content, mucus-binding and metabolic capacities, and host signaling capabilities.
机译：从市售益生菌产品中分离出四种乳酸杆菌菌株，包括来自Vifit（弗里斯兰坎皮纳（Friesland Campina））和Idoform（Ferrosan）的鼠李糖乳杆菌菌株以及来自Actimel（达能）和Yakult（Yakult Honsa Co.）的干酪乳杆菌菌株。他们的基因组和表型进行了表征，并与干酪乳杆菌菌株BL23和鼠李糖乳杆菌GG进行了详细比较。对新分离株的表型分析表明，干酪乳杆菌和鼠李糖乳杆菌菌株在碳水化合物利用上存在差异，这可能与它们的基因型有关。两个分离的鼠李糖乳杆菌菌株具有与鼠李糖乳杆菌GG几乎相同的基因组，证明了它们在食品中的基因组稳定性和完整性。干酪乳杆菌菌株显示出更大的基因组异质性。值得注意的是，所有菌株均包含完整的spaCBA菌毛基因簇。然而，在所测试的条件下，仅鼠李糖乳杆菌菌株产生粘液结合SpaCBA菌毛。转录起始作图表明，在鼠李糖乳杆菌菌株中菌毛基因簇上游插入iso -IS 30元件，但在干酪乳杆菌中不存在，构成了驱动菌毛基因表达的功能启动子。在报告细胞系中，所有鼠李糖乳杆菌菌株均通过Toll样受体2（TLR2）触发了NF-κB反应，而干酪乳杆菌则没有或在较小程度上引起了这种反应。这项研究表明，从益生菌产品中分离出的两种鼠李糖乳杆菌菌株与鼠李糖乳杆菌GG几乎相同，并且进一步突出了这两种菌株与广泛用作益生菌的干酪乳杆菌在基因组含量，黏液结合和代谢方面的差异。容量和主机信令功能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号