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首页> 外文期刊>Applied Microbiology >Abolition of Biofilm Formation in Urinary Tract Escherichia coli and Klebsiella Isolates by Metal Interference through Competition for Fur
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Abolition of Biofilm Formation in Urinary Tract Escherichia coli and Klebsiella Isolates by Metal Interference through Competition for Fur

机译：通过皮草竞争通过金属干扰消除尿道大肠杆菌和克雷伯菌分离物中生物膜的形成。

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摘要

Bacterial biofilms are associated with a large number of persistent and chronic infections. Biofilm-dwelling bacteria are particularly resistant to antibiotics and immune defenses, which makes it hard if not impossible to eradicate biofilm-associated infections. In the urinary tract, free iron is strictly limited but is critical for bacterial growth. Biofilm-associated Escherichia coli cells are particularly desperate for iron. An attractive way of inhibiting biofilm formation is to fool the bacterial regulatory system for iron uptake. Here, we demonstrate that biofilm formation can be impaired by the addition of divalent metal ions, such as Zn(II) and Co(II), which inhibit iron uptake by virtue of their higher-than-iron affinity for the master controller protein of iron uptake, Fur. Reduced biofilm formation of urinary tract-infectious E. coli strains in the presence of Zn(II) was observed in microtiter plates and flow chambers as well as on urinary catheters. These results further support that iron uptake is indeed crucial for biofilm formation, and thereby, targeting these uptake systems might be an effective way to eradicate biofilms caused by infectious strains.
机译：细菌生物膜与大量持续性和慢性感染有关。居住在生物膜上的细菌特别对抗生素和免疫防御系统具有抵抗力，这使得即使不是不可能，也很难根除与生物膜相关的感染。在尿道中，游离铁受到严格限制，但对细菌生长至关重要。与生物膜相关的大肠杆菌细胞特别需要铁。抑制生物膜形成的一种有吸引力的方法是愚弄细菌调节系统以吸收铁。在这里，我们证明可以通过添加二价金属离子（例如Zn（II）和Co（II））来损害生物膜的形成，这些金属离子由于其对铁蛋白的主控制蛋白的亲和力高于铁，从而抑制了铁的吸收。铁摄取，毛皮。在微量滴定板和流动室以及导尿管中，在存在Zn（II）的情况下，尿道感染性大肠杆菌菌株的生物膜形成减少。这些结果进一步支持了铁的摄取对于生物膜的形成确实至关重要，因此，以这些摄取系统为目标可能是根除由感染菌株引起的生物膜的有效方法。

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