...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Influence of Therapeutic Ceftiofur Treatments of Feedlot Cattle on Fecal and Hide Prevalences of Commensal Escherichia coli Resistant to Expanded-Spectrum Cephalosporins, and Molecular Characterization of Resistant Isolates
【24h】

Influence of Therapeutic Ceftiofur Treatments of Feedlot Cattle on Fecal and Hide Prevalences of Commensal Escherichia coli Resistant to Expanded-Spectrum Cephalosporins, and Molecular Characterization of Resistant Isolates

机译：头孢噻呋治疗性肥育对耐扩谱头孢菌种的大肠埃希菌粪便和皮革流行率的影响以及分离株的分子特征

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In the United States, the bla _(CMY-2) gene contained within incompatibility type A/C (IncA/C) plasmids is frequently identified in extended-spectrum-cephalosporin-resistant (ESC~(r)) Escherichia coli strains from both human and cattle sources. Concerns have been raised that therapeutic use of ceftiofur in cattle may increase the prevalence of ESC~(r) E. coli . We report that herd ESC~(r) E. coli fecal and hide prevalences throughout the residency of cattle at a feedlot, including during the period of greatest ceftiofur use at the feedlot, were either not significantly different ( P ≥ 0.05) or significantly less ( P < 0.05) than the respective prevalences at arrival. Longitudinal sampling of cattle treated with ceftiofur demonstrated that once the transient increase of ESC~(r) E. coli shedding that follows ceftiofur injection abated, ceftiofur-injected cattle were no more likely than untreated members of the same herd to shed ESC~(r) E. coli . Pulsed-field gel electrophoresis (PFGE) genotyping, antibiotic resistance phenotyping, screening for presence of the bla _(CMY-2) gene, and plasmid replicon typing were performed on 312 ESC~(r) E. coli isolates obtained during six sampling periods spanning the 10-month residence of cattle at the feedlot. The identification of only 26 unique PFGE genotypes, 12 of which were isolated during multiple sampling periods, suggests that clonal expansion of feedlot-adapted bla _(CMY-2) E. coli strains contributed more to the persistence of bla _(CMY-2) than horizontal transfer of IncA/C plasmids between E. coli strains at this feedlot. We conclude that therapeutic use of ceftiofur at this cattle feedlot did not significantly increase the herd prevalence of ESC~(r) E. coli .
机译：在美国，不相容类型A / C（IncA / C）质粒中包含的bla _（CMY-2）基因经常在两种细菌的​​广谱头孢菌素耐药（ESC〜（r））大肠杆菌菌株中鉴定人和牛的来源。有人担心在牛中头孢噻呋的治疗用途可能会增加ESC〜（r）大肠杆菌的患病率。我们报告说，整个饲养场中牛居住的牛群ESC〜（r）大肠杆菌粪便和皮革流行率，包括在该饲养场中最大头孢噻呋使用期间，均无显着差异（P≥0.05）或显着降低（P <0.05）比到达时的患病率高。对头孢噻呋治疗的牛进行的纵向采样表明，一旦头孢噻呋注射后ESC〜（r）大肠杆菌脱落的瞬时增加减弱，注射头孢噻呋的牛比未处理的同一群牛更不可能脱落ESC〜（r大肠杆菌。脉冲场凝胶电泳（PFGE）基因分型，抗生素抗性表型，bla _（CMY-2）基因的存在筛选和质粒复制子分型在六个采样周期中获得的312株ESC〜（r）大肠杆菌中进行跨越牛在饲养场的10个月居住。仅26种独特的PFGE基因型的鉴定，在多个采样期间分离出其中的12种，这表明饲养场适应型bla _（CMY-2）大肠杆菌菌株的克隆扩增对bla _（CMY-2）的持久性贡献更大。 ），而不是在该饲养场中在大肠杆菌菌株之间进行IncA / C质粒的水平转移。我们得出的结论是，在该牛饲养场中头孢噻呋的治疗性使用不会显着增加ESC〜（r）大肠杆菌的牛群流行率。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号